Title |
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en
Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography
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Creator |
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Accessrights |
open access |
Subject |
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Other
en
Regiospecific analysis
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Other
en
High-performance thin-layer chromatography
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Other
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Triacylglycerol
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Other
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Fatty acid
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Other
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Fish oil
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Other
en
Seal oil
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NDC
660
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Description |
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Abstract
en
This paper presents a smaller-sized procedure for regiospecific analysis of triacylglycerols (TAG) using boric acid high-performance thin-layer chromatography (HPTLC). Cod liver/mackerel, bonito head, and seal oils TAG (2mg) were partially hydrolyzed by ethyl magnesium bromide, and resulting 1(3)-and 2-monoacylglycerols (MAG) were isolated by the HPTLC. Fatty acids of the 1(3)-and 2-MAG were analyzed by gas-liquid chromatography (GLC). Positional distributions of fatty acids in TAG observed for the three marine oils were similar to those previously determined by the procedure requiring 100mg of starting TAG. The new procedure appeared to be usable for regiospecific analysis of a few milligrams of marine oils TAG.
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Publisher |
ja
北海道大学大学院水産科学研究院
en
RESEARCH FACULTY OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY
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Date |
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Language |
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Resource Type |
departmental bulletin paper |
Version Type |
VoR |
Identifier |
HDL
http://hdl.handle.net/2115/32486
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Journal |
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ja
北海道大学水産科学研究彙報
en
BULLETIN OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY
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Volume Number57
Issue Number3
Page Start37
Page End41
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File |
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Oaidate |
2023-07-26 |