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Title
  • en Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography
Creator
    • en Haba, Yusuke
    • en Takase, Kiwamu
    • en Sakai, Shinya
Accessrights open access
Subject
  • Other en Regiospecific analysis
  • Other en High-performance thin-layer chromatography
  • Other en Triacylglycerol
  • Other en Fatty acid
  • Other en Fish oil
  • Other en Seal oil
  • NDC 660
Description
  • Abstract en This paper presents a smaller-sized procedure for regiospecific analysis of triacylglycerols (TAG) using boric acid high-performance thin-layer chromatography (HPTLC). Cod liver/mackerel, bonito head, and seal oils TAG (2mg) were partially hydrolyzed by ethyl magnesium bromide, and resulting 1(3)-and 2-monoacylglycerols (MAG) were isolated by the HPTLC. Fatty acids of the 1(3)-and 2-MAG were analyzed by gas-liquid chromatography (GLC). Positional distributions of fatty acids in TAG observed for the three marine oils were similar to those previously determined by the procedure requiring 100mg of starting TAG. The new procedure appeared to be usable for regiospecific analysis of a few milligrams of marine oils TAG.
Publisher ja 北海道大学大学院水産科学研究院 en RESEARCH FACULTY OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY
Date
    Issued2007-12
Language
  • eng
Resource Type departmental bulletin paper
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/32486
Journal
    • PISSN 1346-1842
      • ja 北海道大学水産科学研究彙報 en BULLETIN OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY
      • Volume Number57 Issue Number3 Page Start37 Page End41
File
    • fulltext P37-41.pdf
    • 161.0 KB (application/pdf)
      • Issued2007-12
Oaidate 2023-07-26