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Title
  • en Development of a 3D collagen scaffold coated with multiwalled carbon nanotubes
Creator
    • en Hirata, Eri
    • en Akasaka, Tsukasa
    • en Watari, Fumio
    • en Yokoyama, Atsuro
Accessrights open access
Rights
  • en This is the pre-peer reviewed version of the following article: Journal of Biomedical Materials Research, Part B, 90B(2), 2009, pp.629-634, which has been published in final form at http://dx.doi.org/10.1002/jbm.b.31327
Subject
  • Other en carbon nanotubes
  • Other en coating
  • Other en collagen sponge honeycomb
  • Other en 3D scaffolds
  • Other en cell culture
  • NDC 497
Description
  • Abstract en Carbon nanotubes (CNTs) have attractive biochemical properties such as strong cell adhesion and protein absorption, which are very useful for a cell cultivation scaffold. In this study, we prepared a multiwalled carbon nanotube-coated collagen sponge (MWCNT-coated sponge) to improve the surface properties of the collagen sponge, and its cell culturing properties were examined. The suface of the collagen sponge was homogeneously coated with MWCNTs by dispersion. MC3T3-E1 cells were cultured on and inside the MWCNT-coated sponge. The DNA content on the MWCNT-coated sponge after 1 week of culture was significantly higher than on an uncoated collagen sponge (p<0.05). There was no significant difference between the estimated ALP activity normalized by DNA quantity on the MWCNT-coated sponge and that on the uncoated collagen sponge which is well known as one of the best scaffolds for cell cultivation. In addition, the MWCNT-coated surface shows strong cell adhesion. Therefore, the MWCNT-coated collagen sponge is expected to be a useful 3D scaffold for cell cultivation.
Publisher en Wiley
Date
    Issued2009-08
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/38894
Relation
  • isVersionOf DOI https://doi.org/10.1002/jbm.b.31327
  • PMID 19213050
Journal
    • PISSN 1552-4973
    • EISSN 1552-4981
    • NCID AA11881538
      • en Journal of Biomedical Materials Research. Part B, Applied Biomaterials
      • Volume Number90 Issue Number2 Page Start629 Page End634
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Oaidate 2023-07-26