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Title
  • ja 加熱損傷ウェルシュ菌の検出培地の比較と改良
  • en Comparison and Improvement of Detection Media for Sub-lethal Injured Clostridium perfringens
Creator
    • ja 清水, 茂雅 en Shimizu, Shigemasa
    • ja 堀口, 明日香 en Horiguchi, Asuka
Accessrights open access
Subject
  • Other en Clostridium perfringens
  • Other en Injured cell
  • Other en Recovery
  • Other en Sodium pyruvate
  • NDC 660
Description
  • Abstract en Recovery of heat-injured vegetative cells and spores of Clostridium perfringens was evaluated on selective media, CW Egg-yolk agar with kanamycin (ECW+A), Tryptose sulfite cycloserine agar(TSC+A)and modified Handford agar (mHFA). As a result of heat treatment at 54℃ for 15 min, viable counts of C. perfringens vegetative cells on a selective medium (ECW+A)was significantly less than those on non-selective media. This means C. perfringens vegetative cells should be in an injured state. Comparing of the three selective media, TSC+A was the best growth and recovery of heat-injured C. perfringens vegetative cells. Recovery on ECW+A was 1 log CFU/ml less than that on TSC+A. Supplementation of TSC+A with sodium pyruvate (0.1-0.5%) further enhanced recovery and detection of heat-injured C. perfringens vegetative cells, and its efficacy was the highest on TSC+A supplemented with 0.3% sodium pyruvate. Supplementation of TSC+A with sodium pyruvate did not affect recovery of heat-injured (95℃, 2 or 60 min) C. perfringens spores. These findings suggest that TSC+A is the most favorable medium for enumeration of C. perfringens and supplementation with sodium pyruvate improves recovery and detection of heat-injured C. perfringens.
Publisher ja 北海道大学大学院水産科学研究院 en Research Faculty of Fisheries Sciences, Hokkaido University
Date
    Issued2009-08-03
Language
  • jpn
Resource Type departmental bulletin paper
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/39007
Journal
    • PISSN 1346-1842
      • ja 北海道大学水産科学研究彙報 en Bulletin of Fisheries Sciences, Hokkaido University
      • Volume Number59 Issue Number2 Page Start37 Page End42
File
    • fulltext 59-2_001.pdf
    • 386.39 KB (application/pdf)
      • Issued2009-08-03
Oaidate 2023-07-26