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Title
  • Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues
Creator

Shetty, Ajaya Kumar

Kobayashi, Takanari

Mizumoto, Shuji

Narumi, Masaki

Kudo, Yoshiaki

Yamada, Shuhei

Sugahara, Kazuyuki

Subject
  • Other Chondroitin sulfate
  • Other Glycosaminoglycan
  • Other Growth factor
  • Other Neurite outgrowth
  • Other Squid liver
  • Other Surface plasmon resonance
  • NDC 464
Description
Other
  • Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6 kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1-3GalNAc(6-O-sulfate), GlcAβ1-3GalNAc(4-O-sulfate), and GlcAβ1-3GalNAc(4,6-O-disulfate) as the major ones, significant amounts of rare 3-O-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1-3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.
PublisherElsevier Ltd.
Date Issued 2009-08-17
Languageeng
NIItypejournal article
VersiontypeAM
Identifier URI http://hdl.handle.net/2115/39341
Relation
  • isIdenticalTo PMID 19344892
  • isIdenticalTo DOI https://doi.org/10.1016/j.carres.2009.02.029
Journal
    • ISSN 0008-6215
    • Carbohydrate Research
    344(12), 1526-1532
File
Oaidate2019-03-15T05:20:32Z