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Title
  • en Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood
Creator
Accessrights open access
Subject
  • Other en Bacteriocin
  • Other en Pediocin
  • Other en Pediococcus
  • Other en Medium components
  • Other en Culture condition
  • Other en Optimization
  • NDC 660
Description
  • Abstract en Growth requirements for maximum production of pediocin Iz3.13 by Pediococcus pentosaceus Iz3.13 were studied. Physical and chemical parameters that enhanced pediocin production included incubation in static broth at 30℃, with an initial pH of 4.5. Optimum nutritional conditions that enhanced pediocin Iz3.13 production were 3.5% Trypticase peptone, 3.5% Phytone peptone or 5.0% Proteose peptone No.3 as nitrogen sources, and glucose or maltose as carbon sources. Supplementing growth media with beef extract and MgCl2 also enhanced production. This study proposes an optimized growth medium containing 5.0% Proteose peptone No.3, 3.5% Phytone peptone, 2.5% glucose, 1.5% MgCl2, 3.0% beef extract and 0.25% K2HPO4. This medium significantly enhanced pediocin Iz3.13 production (5.1 fold increase) as compared to a basal medium.
Publisher ja 北海道大学大学院水産科学研究院 en Research Faculty of Fisheries Sciences, Hokkaido University
Date
    Issued2010-03-09
Language
  • eng
Resource Type departmental bulletin paper
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/42764
Journal
    • PISSN 1346-1842
      • ja 北海道大学水産科学研究彙報 en Bulletin of Fisheries Sciences, Hokkaido University
      • Volume Number60 Issue Number1 Page Start5 Page End12
File
Oaidate 2023-07-26