Title |
-
en
Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood
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Creator |
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Accessrights |
open access |
Subject |
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Other
en
Bacteriocin
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Other
en
Pediocin
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Other
en
Pediococcus
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Other
en
Medium components
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Other
en
Culture condition
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Other
en
Optimization
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NDC
660
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Description |
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Abstract
en
Growth requirements for maximum production of pediocin Iz3.13 by Pediococcus pentosaceus Iz3.13 were studied. Physical and chemical parameters that enhanced pediocin production included incubation in static broth at 30℃, with an initial pH of 4.5. Optimum nutritional conditions that enhanced pediocin Iz3.13 production were 3.5% Trypticase peptone, 3.5% Phytone peptone or 5.0% Proteose peptone No.3 as nitrogen sources, and glucose or maltose as carbon sources. Supplementing growth media with beef extract and MgCl2 also enhanced production. This study proposes an optimized growth medium containing 5.0% Proteose peptone No.3, 3.5% Phytone peptone, 2.5% glucose, 1.5% MgCl2, 3.0% beef extract and 0.25% K2HPO4. This medium significantly enhanced pediocin Iz3.13 production (5.1 fold increase) as compared to a basal medium.
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Publisher |
ja
北海道大学大学院水産科学研究院
en
Research Faculty of Fisheries Sciences, Hokkaido University
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Date |
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Language |
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Resource Type |
departmental bulletin paper |
Version Type |
VoR |
Identifier |
HDL
http://hdl.handle.net/2115/42764
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Journal |
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-
ja
北海道大学水産科学研究彙報
en
Bulletin of Fisheries Sciences, Hokkaido University
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Volume Number60
Issue Number1
Page Start5
Page End12
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File |
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Oaidate |
2023-07-26 |