| Title |
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en
Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix
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| Creator |
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| Accessrights |
open access |
| Subject |
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Other
en
Lung adenocarcinoma
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Other
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Irradiation
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Other
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3D culture
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Other
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Collagen gel
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Other
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Invasion
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Other
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Integrin β1
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NDC
492
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| Description |
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Abstract
en
Radiotherapy is one of the effective therapies used for treating various malignant tumors. However, the emergence of tolerant cells after irradiation remains problematic due to their high metastatic ability, sometimes indicative of poor prognosis. In this study, we showed that subcloned human lung adenocarcinoma cells (A549P-3) that are irradiation-tolerant indicate high invasive activity in vitro, and exhibit an integrin β1 activity-dependent migratory pattern. In collagen gel overlay assay, majority of the A549P-3 cells displayed round morphology and low migration activity, whereas a considerable number of A549P-3IR cells surviving irradiation displayed a spindle morphology and high migration rate. Blocking integrin β1 activity reduced the migration rate of A549P-3IR cells and altered the cell morphology allowing them to assume a round shape. These results suggest that the A549P-3 cells surviving irradiation acquire a highly invasive integrin β1-dependent phenotype, and integrin β1 might be a potentially effective therapeutic target in combination with radiotherapy.
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| Publisher |
en
Elsevier
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
AM |
| Identifier |
HDL
http://hdl.handle.net/2115/43148
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| Relation |
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isVersionOf
DOI
https://doi.org/10.1016/j.bbrc.2010.04.150
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PMID
20438698
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| Journal |
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en
Biochemical and Biophysical Research Communications
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Volume Number396
Issue Number3
Page Start651
Page End655
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| File |
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| Oaidate |
2023-07-26 |
