Enzyme-Linked Immunosorbent Assay for Detection of Filovirus Species-Specific Antibodies

Nakayama Eri; Yokoyama Ayaka; Miyamoto Hiroko; Igarashi Manabu; Kishida Noriko; Matsuno Keita; Marzi Andrea; Feldmann Heinz; Ito Kimihito; Saijo Masayuki; Takada Ayato

Title	en Enzyme-Linked Immunosorbent Assay for Detection of Filovirus Species-Specific Antibodies
Creator	en Nakayama, Eri en Yokoyama, Ayaka en Miyamoto, Hiroko en Igarashi, Manabu NRID 1000010374240 en Kishida, Noriko NRID 1000070435965 en Matsuno, Keita en Marzi, Andrea en Feldmann, Heinz en Ito, Kimihito NRID 1000060396314 en Saijo, Masayuki NRID 1000050300926 en Takada, Ayato NRID 1000010292062
Accessrights	open access
Rights	en Copyright © 2010, American Society for Microbiology
Subject	NDC 491
Description	Abstract en Several enzyme-linked immunosorbent assays (ELISA) for the detection of filovirus-specific antibodies have been developed. However, diagnostic methods to distinguish antibodies specific to the respective species of filoviruses, which provide the basis for serological classification, are not readily available. We established an ELISA using His-tagged secreted forms of the transmembrane glycoproteins (GPs) of five different Ebola virus (EBOV) species and one Marburg virus (MARV) strain as antigens for the detection of filovirus species-specific antibodies. The GP-based ELISA was evaluated by testing antisera collected from mice immunized with virus-like particles as well as from humans and nonhuman primates infected with EBOV or MARV. In our ELISA, little cross-reactivity of IgG antibodies was observed in most of the mouse antisera. Although sera and plasma from some patients and monkeys showed notable cross-reactivity with the GPs from multiple filovirus species, the highest reactions of IgG were uniformly detected against the GP antigen homologous to the virus species that infected individuals. We further confirmed that MARV-specific IgM antibodies were specifically detected in specimens collected from patients during the acute phase of infection. These results demonstrate the usefulness of our ELISA for diagnostics as well as ecological and serosurvey studies.
Publisher	en American Society for Microbiology
Date	Issued2010-11
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/45376
Relation	isVersionOf DOI https://doi.org/10.1128/CVI.00170-10
Journal	PISSN 1556-6811 en Clinical and Vaccine Immunology Volume Number17 Issue Number11 Page Start1723 Page End1728
File	fulltext CVI17-11_1723-1728.pdf 457.94 KB (application/pdf) Issued2010-11
Oaidate	2023-07-26