Back

Title
  • en High expression of the mRNA of cytochrome P450 and phase II enzymes in the lung and kidney tissues of cattle
Creator
    • en Darwish, W. S.
    • en Ikenaka, Y.
    • en El-Ghareeb, W. R.
Accessrights open access
Rights
  • en © The Animal Consortium 2010
Subject
  • Other en cytochrome P450
  • Other en phase II enzymes
  • Other en cattle
  • Other en tissue distribution
  • NDC 649
Description
  • Abstract en The objective of this study was to investigate the tissue-specific mRNA expression of different cytochrome P450 (CYP) isoforms, UDP glucuronsyl transferase 1A1 (UGT1A1) and glutathione-S-transferase (GSTA1) in the different tissues (liver, mammary gland, lungs, spleen, kidney cortex, heart, masseter muscle and tongue) of cattle, using quantitative real-time polymerase chain reaction (qPCR). CYP1A1-like mRNA was expressed in all of the tissues examined, including the liver, with the highest expression level in the kidney. CYP1A2-, 2E1- and 3A4-like mRNAs were only expressed hepatically. Interestingly, significant expression of CYP2B6-like mRNA was recorded in the lung tissue, while CYP2C9-like mRNA was expressed in the liver and kidney tissues of the cattle examined. UGT1A1- and GSTA1-like mRNAs were expressed in all of the examined tissues, except the mammary glands, and the highest expression levels were recorded in the kidney. The high expression of UGT1A1 in the lung tissue and GSTA1 in the liver tissue was unique to cattle; this has not been reported for rats or mice. The findings of this study strongly suggest that the liver, kidneys and lungs of cattle are the major organs contributing to xenobiotics metabolism.
Publisher en Cambridge University Press
Date
    Issued2010-12-01
Language
  • eng
Resource Type journal article
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/47523
Relation
  • URI http://journals.cambridge.org/action/displayJournal?jid=ANM
  • isIdenticalTo DOI https://doi.org/10.1017/S1751731110001394
  • PMID 22445377
Journal
    • PISSN 1751-7311
      • en Animal
      • Volume Number4 Issue Number12 Page Start2023 Page End2029
File
Oaidate 2023-07-26