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Title
  • en Temperature controls nuclear import of Tam3 transposase in Antirrhinum
Creator
    • en Fujino, Kaien
    • en Hashida, Shin-nosuke
    • en Ogawa, Takashi
    • en Natsume, Tomoko
    • en Uchiyama, Takako
    • en Mikami, Tetsuo
Accessrights open access
Rights
  • en The definitive version is available at www.blackwell-synergy.com
Subject
  • Other en low-temperature-dependent transposition (LTDT)
  • Other en nuclear import
  • Other en transposase (TPase)
  • Other en transposon Tam3
  • Other en Antirrhinum majus
  • NDC 471
Description
  • Abstract en It has been proposed that environmental stimuli can activate transposable elements (TEs), while few substantial mechanisms have been shown so far. The class II element Tam3 from Antirrhinum majus exhibits a unique property of low-temperature-dependent transposition (LTDT). LTDT has proved invaluable in developing the gene isolation technologies that have underpinned much of modern plant developmental biology. Here, we reveal that LTDT involves differential sub-cellular localization of the Tam3 transposase (TPase) in cells grown at low (15℃) and high (25℃) temperatures. The mechanism is associated with the nuclear import of Tam3 TPase in Antirrhinum cells. At high temperature, the nuclear import of Tam3 TPase is severely restricted in Antirrhinum cells, while at low temperature, the nuclear localization of Tam3 TPase is observed in about 20% of the cells. However, in tobacco BY-2 and onion cells, Tam3 TPase is transported into most nuclei. In addition to three nuclear localization signals (NLSs), the Tam3 TPase is equipped with a nuclear localization inhibitory domain (NLID), which functions to abolish nuclear import of the TPase at high temperature in Antirrhinum. NLID in Tam3 TPase is considered to interact with Antirrhinum specific factor(s). The host-specific regulation of the nuclear localization of transposase represents a new repertoire controlling class II TEs.
Publisher en Wiley-Blackwell
Date
    Issued2011-01
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/47949
Relation
  • isVersionOf DOI https://doi.org/10.1111/j.1365-313X.2010.04405.x
  • PMID 21175897
Journal
    • PISSN 0960-7412
      • en The Plant Journal
      • Volume Number65 Issue Number1 Page Start146 Page End155
File
Oaidate 2023-07-26