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Title
  • en Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5
Creator
Accessrights open access
Subject
  • Other en Omsk hemorrhagic fever
  • Other en Flavivirus
  • Other en Infectious cDNA
  • Other en Viral replication
  • NDC 491
Description
  • Abstract en Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis serocomplex of flaviviruses, and causes hemorrhagic disease in humans. In this study, an infectious cDNA of OHFV was constructed to investigate the molecular mechanisms involved in OHFV pathogenesis for the first time. Our cDNA clone was capable of producing infectious virus which is genetically identical to the parental Guriev strain, and the recombinant virus showed similar biological properties to the parental virus including growth kinetics and virulence characteristics. While characterizing the cDNAs, fortuitous mutations at NS2A position 46 and NS5 position 836 were found to affect viral production. By using a viral replicon expressing luciferase, it was shown that both of the mutations produced a defect in RNA replication and that the NS5 mutation induced a temperature-sensitive phenotype, indicating the importance of these residues in RNA replication. This infectious cDNA will be a useful tool to study the replication and pathogenesis of OHFV.
Publisher en Elsevier B.V.
Date
    Issued2011-01
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/45025
Relation
  • isVersionOf DOI https://doi.org/10.1016/j.virusres.2010.08.023
  • PMID 20817056
Journal
    • PISSN 0168-1702
      • en Virus Research
      • Volume Number155 Issue Number1 Page Start61 Page End68
File
Oaidate 2023-07-26