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Title
  • en Occurrence of all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3) in roughscale sole Clidoderma asperrimum flesh lipids
  • ja サメガレイClidoderma asperrimum の筋肉脂質における全-cis-5,8,11,14,17,20,23-ヘキサコサヘプタエン酸（26:7n-3）の存在
Creator
    • en Fukuda, Yuuki ja 福田, 悠紀
Accessrights open access
Rights
  • ja © 2011 公益社団法人日本水産学会
  • en © 2011 The Japanese Society of Fisheries Science
  • en The final publication is available at www.springerlink.com
Subject
  • Other en Hexacosaheptaenoic acid
  • Other en Very long chain polyunsaturated fatty acid
  • Other en Fatty acid
  • Other en Roughscale sole
  • Other en Clidoderma asperrimum
  • Other en GC
  • Other ja GC–MS
  • NDC 464
Description
  • Abstract en Fatty acid analysis of roughscale sole Clidoderma asperrimum flesh lipids was carried out by gas chromatography. An unidentified peak appeared in the chromatogram in the elution region of ≥C24 fatty acids. After enrichment by solvent partitioning, reversed-phase thin-layer chromatography (TLC), and argentation TLC, the peak component was subjected to structural analyses. The partially hydrogenated products after reaction with hydrazine hydrate gave seven isomers of cis-hexacosenoic acid (26:1). Gas chromatography-mass spectrometry (GC–MS) analyses of their dimethyl disulfide adducts identified the monounsaturates as 5-, 8-, 11-, 14-, 17-, 20-, and 23-26:1. The peak component was assigned to all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3). GC–MS analyses of the 4,4-dimethyloxazoline derivative and methyl ester confirmed this structure. This fatty acid is a rare, very long-chain polyunsaturated fatty acid (VLCPUFA). The concentrations of the acid found in roughscale sole were 0.69 ± 0.34% (N = 5) of total fatty acids in flesh lipids. Roughscale sole appears to be characterized by the occurrence of 26:7n-3, which is lacking in popular sources of methylene-interrupted VLCPUFA, such as vertebrate retina, spermatozoa, and herring.
  • Abstract ja 北海道産サメガレイの筋肉脂質の脂肪酸をGC で分析したところ，炭素数24 以上の領域に未知のピークが出現した。この成分を濃縮後，数種の誘導体に変換してGC-MSに供した結果，同成分は全-cis-5,8,11,14,17,20,23-ヘキサコサヘプタエン酸（26:7n-3）と同定された。総脂肪酸中の含有量は供試5 個体の平均で0.69±0.34％であった。サメガレイは，n-3 系およびn-6 系超長鎖ポリエン酸の給源として一般的な高等動物の網膜，精子，ニシン油などには見られない26:7n-3 を含む点で特徴的である。
Publisher en Japanese Society of Fisheries Science
Date
    Issued2011-09
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/47167
Relation
  • isVersionOf DOI https://doi.org/10.1007/s12562-011-0387-y
Journal
    • PISSN 0919-9268
    • EISSN 1444-2906
    • NCID AA10993718
      • en Fisheries Science
      • Volume Number77 Issue Number5 Page Start875 Page End882
File
Oaidate 2023-10-14