| Title |
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Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection
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| Creator |
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| Accessrights |
open access |
| Subject |
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Other
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Chondroitin sulfate E
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Other
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Japanese encephalitis virus
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Other
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Neural cell
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NDC
465
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| Description |
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Abstract
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Glycosaminoglycans (GAGs) have diverse functions in the body and are involved in viral infection. The purpose of this study was to evaluate the possible roles of the E-disaccharide units GlcAβ1-3GalNAc(4,6-O-disulfate) of chondroitin sulfate (CS), a GAG involved in neuritogenesis and neuronal migration, in Japanese encephalitis virus (JEV) infection. Soluble CS-E (sCS-E) derived from squid cartilage inhibited JEV infection in African green monkey kidney-derived Vero cells and baby hamster kidney-derived BHK cells by interfering with viral attachment. In contrast, sCS-E enhanced viral infection in the mouse neuroblastoma cell line Neuro-2a, despite the fact that viral attachment to Neuro-2a cells was inhibited by sCS-E. This enhancement effect in Neuro-2a cells seemed to be related to increased viral RNA replication and was also observed in a rat infection model in which intracerebral coadministration of sCS-E with JEV in 17-day-old rats resulted in higher brain viral loads than in rats infected without sCS-E administration. These results show the paradoxical effects of sCS-E on JEV infection in different cell types and indicate that potential use of sCS-E as an antiviral agent against JEV infection should be approached with caution considering its effects in the neuron, the major target of JEV.
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| Publisher |
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Elsevier
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
AM |
| Identifier |
HDL
http://hdl.handle.net/2115/46843
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| Relation |
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isVersionOf
DOI
https://doi.org/10.1016/j.bbrc.2011.05.072
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PMID
21621516
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| Journal |
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Biochemical and Biophysical Research Communications
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Volume Number409
Issue Number4
Page Start717
Page End722
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| File |
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| Oaidate |
2023-07-26 |
