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Title
  • en Application of PTR-MS to an incubation experiment of the marine diatom Thalassiosira pseudonana
Creator
Accessrights open access
Subject
  • Other en dimethyl sulfide
  • Other en isoprene
  • Other en methanethiol
  • Other en PTR-MS
  • Other en Thalassiosira pseudonana
  • Other en axenic culture
  • NDC 452
Description
  • Abstract en Emission of trace gases from the marine diatom Thalassiosira pseudonana (CCMP 1335) was continuously monitored with a proton transfer reaction-mass spectrometry (PTR-MS) in an axenic batch culture system under a 13:11-h light:dark cycle. Substantial increases in the signals at m/z 49, 63, and 69, attributable to methanethiol, dimethyl sulfide (DMS), and isoprene, respectively, were observed in response to increases in cell density. Signals at m/z 69 showed diurnal variations throughout the experiment whereas those at m/z 49 were more pronounced at the beginning of the incubation. Interestingly, the signals at m/z 49 and 69 changed immediately following the light-dark and dark-light transitions, suggesting that light plays a crucial role in the production of methanethiol and isoprene. However, in the latter half of the experiment, methanethiol showed negligible diurnal variations regardless of light conditions, suggesting the production of methanethiol from enzymatic cleavage of DMS. The trend ill signals at m/z 63 was similar to that of the abundance of senescent cells plus cell debris rather than vegetative cells. The results suggest that aging or death of phytoplankton cells could also substantially control DMS production in natural waters along with the other microbial processes related to bacteria and zooplankton.
Publisher en Geochemical Society of Japan
Date
    Issued2011
Language
  • eng
Resource Type journal article
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/50151
Journal
    • PISSN 0016-7002
      • en Geochemical Journal
      • Volume Number45 Issue Number5 Page Start355 Page End363
File
Oaidate 2023-07-26