β-Glucan Derived from Aureobasidium pullulans Is Effective for the Prevention of Influenza in Mice

Muramatsu Daisuke; Iwai Atsushi; Aoki Shiho; Uchiyama Hirohumi; Kawata Koji; Nakayama Yosuke; Nikawa Yasuhiro; Kusano Kisato; Okabe Mitsuyasu; Miyazaki Tadaaki

Title	en β-Glucan Derived from Aureobasidium pullulans Is Effective for the Prevention of Influenza in Mice
Creator	en Muramatsu, Daisuke en Iwai, Atsushi en Aoki, Shiho en Uchiyama, Hirohumi en Kawata, Koji en Nakayama, Yosuke en Nikawa, Yasuhiro en Kusano, Kisato en Okabe, Mitsuyasu en Miyazaki, Tadaaki NRID 1000060272431
Accessrights	open access
Rights	http://creativecommons.org/licenses/by/3.0/ en Creative Commons Attribution 3.0 Unported
Subject	NDC 491
Description	Abstract en β-(1→3)-D-glucans with β-(1→6)-glycosidic linked branches produced by mushrooms, yeast and fungi are known to be an immune activation agent, and are used in anti-cancer drugs or health-promoting foods. In this report, we demonstrate that oral administration of Aureobasidium pullulans-cultured fluid (AP-CF) enriched with the β-(1→3),(1→6)-D-glucan exhibits efficacy to protect mice infected with a lethal titer of the A/Puerto Rico/8/34 (PR8; H1N1) strain of influenza virus. The survival rate of the mice significantly increased by AP-CF administration after sublethal infection of PR8 virus. The virus titer in the mouse lung homogenates was significantly decreased by AP-CF administration. No significant difference in the mRNA expression of inflammatory cytokines, and in the population of lymphocytes was observed in the lungs of mice administered with AP-CF. Interestingly, expression level for the mRNA of virus sensors, RIG-I (retinoic acid-inducible gene-I) and MDA5 (melanoma differentiation-associated protein 5) strongly increased at 5 hours after the stimulation of A. pullulans-produced purified β-(1→3),(1→6)-D-glucan (AP-BG) in murine macrophage-derived RAW264.7 cells. Furthermore, the replication of PR8 virus was significantly repressed by pre-treatment of AP-BG. These findings suggest the increased expression of virus sensors is effective for the prevention of influenza by the inhibition of viral replication with the administration of AP-CF.
Publisher	en Public Library of Science
Date	Issued2012-07-23
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/50063
Relation	isIdenticalTo DOI https://doi.org/10.1371/journal.pone.0041399
Journal	PISSN 1932-6203 en PLoS One Volume Number7 Issue Number7 Page Starte41399
File	fulltext PLoO7-7_e41399.pdf 480.28 KB (application/pdf) Issued2012-07-23
Oaidate	2023-07-26