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Title
  • en Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3
Creator
    • en Kimoto, Mai
    • en Kitagawa, Tsuyuki
    • en Kobayashi, Isao
    • en Nakata, Tomohiro
Accessrights open access
Rights
  • en The original publication is available at www.springerlink.com
Subject
  • Other en Bombyx mori
  • Other en Sericin-1
  • Other en Silk gene
  • Other en Silk gland
  • Other en Homeodomain
  • Other en Gene gun
  • NDC 467
Description
  • Abstract en The sericin-1 gene encoding a glue protein is expressed in the middle silk gland (MSG) of the silkworm, Bombyx mori. A member of the class III POU domain transcription factors, POU-M1, was cloned as the factor bound to the SC site of the sericin-1 promoter and has been proposed to be a positive transcription factor. In this study, we analyzed the expression pattern of the POU-M1 gene in fourth and fifth instars in comparison with the pattern of the sericin-1 gene. The POU-M1 gene was expressed strongly in the region anterior to the sericin-1-expressing portion of the silk gland at both feeding stages. As the sericin-1-expressing region expands from the posterior to middle portions of the MSG in the fifth instar, the POU-M1-expressing region retreated from the middle to anterior portion. Introduction of the expression vector of POU-M1 into the silk glands by gene gun technology repressed promoter activity of the sericin-1 gene, suggesting that POU-M1 regulates the sericin-1 gene negatively. An in vitro binding assay showed that POU-M1 bound not only to the SC site but also to other promoter elements newly detected in vivo. Another spatiotemporal specific factor MIC binds to these elements, and POU-M1 competed with MIC to bind at the -70 site essential for promoter activity. These results suggest that POU-M1 is involved in restricting the anterior boundary of the sericin-1-expressing region in the silk gland by inhibiting the binding of the transcriptional activator to the promoter elements.
Publisher en Springer-Verlag
Date
    Issued2012-11
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/53467
Relation
  • isVersionOf DOI https://doi.org/10.1007/s00427-012-0418-4
  • PMID 23070540
Journal
    • PISSN 0949-944X
      • en Development Genes and Evolution
      • Volume Number222 Issue Number6 Page Start351 Page End359
File
Oaidate 2023-07-26