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Title	en Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses
Creator	en Okamatsu, Masatoshi NRID 1000000507163 en Feng, Fei en Ohyanagi, Tatsuya en Nagahori, Noriko NRID 1000090372268 en Someya, Kazuhiko en Sakoda, Yoshihiro NRID 1000040333637 en Miura, Nobuaki NRID 1000080372267 en Nishimura, Shin-Ichiro NRID 1000000183898 en Kida, Hiroshi NRID 1000010109506
Accessrights	open access
Subject	Other en Influenza virus Other en Hemagglutinin Other en Fluorescence polarization Other en Quantum dot Other en High-throughput screening NDC 649
Description	Abstract en Attachment of influenza virus to susceptible cells is mediated by viral protein hemagglutinin (HA), which recognizes cell surface glycoconjugates that terminate in α-sialosides. To develop anti-influenza drugs based on inhibition of HA-mediated infection, novel fluorescent nanoparticles displaying multiple biantennary N-glycan chains with α-sialosides (A2-PC-QDs) that have high affinity for the HA were designed and constructed. The A2-PC-QDs enabled an easy and efficient fluorescence polarization (FP) assay for detection of interaction with the HA and competitive inhibition even by small molecule compounds against A2-PC-QDs-HA binding. The quantum dot (QD)-based FP assay established in the present study is a useful tool for high-throughput screening and to accelerate the development of novel and more effective blockers of the viral attachment of influenza virus.
Publisher	en Elsevier B.V.
Date	Issued2013-02
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/52274
Relation	isVersionOf DOI https://doi.org/10.1016/j.jviromet.2012.11.004 PMID 23164994
Journal	PISSN 0166-0934 en Journal of Virological Methods Volume Number187 Issue Number2 Page Start390 Page End394
File	fulltext JVM187-2_390-394.pdf 465.29 KB (application/pdf) Issued2013-02
Oaidate	2023-07-26