| Title |
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en
A low pathogenic H5N2 influenza virus isolated in Taiwan acquired high pathogenicity by consecutive passages in chickens.
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| Creator |
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| Accessrights |
open access |
| Subject |
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MeSH
en
Age Factors
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MeSH
en
Amino Acid Sequence
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MeSH
en
Animals
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MeSH
en
Antibodies, Viral/blood
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MeSH
en
Chickens
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MeSH
en
Genome, Viral
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MeSH
en
Influenza A Virus, H5N1 Subtype/genetics
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MeSH
en
Influenza A Virus, H5N1 Subtype/pathogenicity
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MeSH
en
Influenza in Birds/genetics
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MeSH
en
Influenza in Birds/virology
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MeSH
en
Mutation
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MeSH
en
RNA, Viral/chemistry
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MeSH
en
RNA, Viral/genetics
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MeSH
en
Reverse Transcriptase Polymerase Chain Reaction/veterinary
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MeSH
en
Taiwan
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MeSH
en
Virulence
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NDC
491
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| Description |
-
Abstract
en
H5N2 viruses were isolated from cloacal swab samples of apparently healthy chickens in Taiwan in 2003 and 2008 during surveillance of avian influenza. Each of the viruses was eradicated by stamping out. The official diagnosis report indicated that the Intravenous Pathogenicity Indexes (IVPIs) of the isolates were 0.00 and 0.89, respectively, indicating that these were low pathogenic strains, although the hemagglutinin of the strain isolated in 2008 (Taiwan08) had multibasic amino acid residues at the cleavage site (PQRKKR/G). In the present study, these H5N2 viruses were assessed for their intravenous and intranasal pathogenicity for chickens. It was examined whether Taiwan08 acquires pathogenicity through consecutive passages in chickens. Intravenous pathogenicity of Taiwan08 depended upon the age of the chickens used for the IVPI test; all of the eight-week-old chickens intravenously inoculated with Taiwan08 showed clinical signs but survived for ten days post inoculation (IVPI=0.68), whereas all the six-week-old chickens died (IVPI=1.86). Taiwan08-P8, which were passaged in chickens for eight times, killed all the eight-week-old chickens (IVPI=2.36). The four-week-old chickens died after intranasal inoculation of Taiwan08-P8, indicating that Taiwan08 must have become highly pathogenic during circulation in chicken flocks. These results emphasize the importance of a stamping out policy for avian influenza even if the IVPI of the causal virus is low.
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| Publisher |
en
the Japanese Society of Veterinary Science
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
VoR |
| Identifier |
HDL
http://hdl.handle.net/2115/53279
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| Relation |
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isIdenticalTo
DOI
https://doi.org/10.1292/jvms.10-0532
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PMID
21301183
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| Journal |
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en
The Journal of veterinary medical science
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Volume Number73
Issue Number6
Page Start767
Page End772
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| File |
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| Oaidate |
2023-07-26 |
