Evaluation of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a screening method for the detection of influenza viruses in the fecal materials of water birds.

Yoshida Hiromi; Sakoda Yoshihiro; Endo Mayumi; Motoshima Masayuki; Yoshino Fumi; Yamamoto Naoki; Okamatsu Masatoshi; Soejima Takahiro; Senba Syouhei; Kanda Hidetoshi; Kida Hiroshi

Title	en Evaluation of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a screening method for the detection of influenza viruses in the fecal materials of water birds.
Creator	en Yoshida, Hiromi en Sakoda, Yoshihiro NRID 1000040333637 en Endo, Mayumi en Motoshima, Masayuki en Yoshino, Fumi en Yamamoto, Naoki en Okamatsu, Masatoshi NRID 1000000507163 en Soejima, Takahiro en Senba, Syouhei en Kanda, Hidetoshi en Kida, Hiroshi NRID 1000010109506
Accessrights	open access
Subject	MeSH en Animals MeSH en Disease Reservoirs/veterinary MeSH en Ducks MeSH en Feces/virology MeSH en Influenza A Virus, H3N2 Subtype/genetics MeSH en Influenza A Virus, H3N2 Subtype/isolation & purification MeSH en Influenza in Birds/diagnosis MeSH en Influenza in Birds/virology MeSH en Nucleic Acid Amplification Techniques/methods MeSH en Nucleic Acid Amplification Techniques/veterinary MeSH en Phylogeny MeSH en RNA, Viral/chemistry MeSH en RNA, Viral/genetics MeSH en Reverse Transcription MeSH en Sensitivity and Specificity MeSH en Viral Matrix Proteins/chemistry MeSH en Viral Matrix Proteins/genetics NDC 491
Description	Abstract en Migratory water birds are a natural reservoir for influenza A viruses. Viruses replicate in the intestines of ducks and are shed with the fecal materials. Virus isolation from collected fecal materials, therefore, is an integral part of the surveillance of avian influenza in water birds. In the present study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was assessed for its usefulness in detecting the RNA of influenza A viruses in fecal materials. It was found that, RT-LAMP specifically and sensitively detects the matrix gene of influenza A viruses. Influenza A viruses were isolated from the fecal materials in which viral RNA were detected by RT-LAMP in 35 min. The present findings indicate that RT-LAMP is useful as a high throughput screening method for field samples prior to virus isolation, allowing the processing of hundreds of samples per day.
Publisher	en the Japanese Society of Veterinary Science
Date	Issued2011-06
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/53281
Relation	isIdenticalTo DOI https://doi.org/10.1292/jvms.10-0505 PMID 21289470
Journal	PISSN 1347-7439 en The Journal of veterinary medical science Volume Number73 Issue Number6 Page Start753 Page End758
File	fulltext 73_753.pdf 514.14 KB (application/pdf) Issued2011-06
Oaidate	2023-07-26