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Title
  • en Characterization of phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species : Unique pyrene metabolism and species specificity observed in fish species
Creator
Accessrights open access
Subject
  • Other en Fish
  • Other en Pyrene
  • Other en Conjugation
  • Other en Polycyclic aromatic hydrocarbons (PAHs)
  • Other en Metabolism
  • Other en Inter-species difference
  • NDC 649
Description
  • Abstract en Metabolic activity, particularly conjugation, was examined in fish by analyzing pyrene (a four-ring, polycyclic aromatic hydrocarbon) metabolites using high-performance liquid chromatography (HPLC) with fluorescence detector (FD), a mass spectrometry (MS) system, and kinetic analysis of conjugation enzymes. Fourteen fresh water fish species, including Danio rerio and Orizias latipes, were exposed to aqueous pyrene, and the resulting metabolites were collected. Identification of pyrene metabolites by HPLC/FD and ion-trap MS indicated that the major metabolites were pyrene glucuronide and pyrene sulfate in all 14 species. Differences were observed in pyrene glucuronide:pyrene sulfate ratio and in the total amount of pyrene conjugates excreted between fish species. Furthermore, a correlation was found between the amount of pyrene glucuronide present and the total amount of the pyrene metabolite eliminated. Kinetic analysis of conjugation by hepatic microsomes in vitro indicated that the differences in excreted metabolites reflected the differences in enzymatic activities. (C) 2013 Elsevier B.V. All rights reserved.
Publisher en Elsevier
Date
    Issued2013-09
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/53434
Relation
  • isVersionOf DOI https://doi.org/10.1016/j.etap.2013.05.018
  • PMID 23834960
Journal
    • PISSN 1382-6689
      • en Environmental Toxicology and Pharmacology
      • Volume Number36 Issue Number2 Page Start567 Page End578
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Oaidate 2023-07-26