Title |
-
en
Comparison of the compositions of phospholipid-associated fatty acids in wild-type and extracellular matrix tenascin-X-deficient mice.
|
Creator |
|
Accessrights |
open access |
Subject |
-
Other
en
tenascin-X
-
Other
en
phospholipid
-
Other
en
triglyceride
-
Other
en
fatty acid
-
Other
en
phosphatidylcholine
-
Other
en
phosphatidylethanolamine
-
MeSH
en
Animals
-
MeSH
en
Cell Line
-
MeSH
en
Fatty Acids/metabolism
-
MeSH
en
Fibroblasts/metabolism
-
MeSH
en
Gene Expression Regulation/genetics
-
MeSH
en
Gene Expression Regulation/physiology
-
MeSH
en
Mice
-
MeSH
en
Mice, Knockout
-
MeSH
en
Peripheral Nerves/metabolism
-
MeSH
en
Phospholipids/metabolism
-
MeSH
en
Subcutaneous Tissue/metabolism
-
MeSH
en
Tenascin/deficiency
-
MeSH
en
Tenascin/genetics
-
MeSH
en
Tenascin/physiology
-
NDC
499
|
Description |
-
Abstract
en
Tenascin-X (TNX) is a member of the tenascin family of glycoproteins of the extracellular matrix. We previously showed that TNX regulates the synthesis of triglyceride and the composition of triglyceride-associated fatty acids. The aim of the present study was to determine whether TNX controls the synthesis of phospholipids and the composition of phospholipid-associated fatty acids by using TNX-deficient (TNX-/-) mice and TNX-overexpressing fibroblast cell lines. Thin-layer chromatography of total lipids of the skin and sciatic nerves from wild-type and TNX-/- mice revealed that the amounts of major phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in wild-type and TNX-/- mice are not different. Gas chromatography-mass spectrometry showed that the major fatty acid compositions of PC and PE in wild-type and TNX-/- mice are almost the same. Fibroblast cells stably overexpressing TNX also showed almost the same amounts of PC and PE and almost the same fatty acid compositions of PC and PE as those in mock-transfected cells. These results suggest that TNX regulates the amount of triglyceride and the composition of triglyceride-associated fatty acids but not the amount and species of phospholipids or the composition of phospholipid-associated fatty acids.
|
Publisher |
en
The Pharmaceutical Society of Japan
|
Date |
|
Language |
|
Resource Type |
journal article |
Version Type |
VoR |
Identifier |
HDL
http://hdl.handle.net/2115/53740
|
Relation |
-
isIdenticalTo
DOI
https://doi.org/10.1248/bpb.27.1447
-
PMID
15340236
|
Journal |
-
-
en
Biological & pharmaceutical bulletin
-
Volume Number27
Issue Number9
Page Start1447
Page End1450
|
File |
|
Oaidate |
2023-07-26 |