Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene.

Saitoh Yasushi; Miyagi Satoru; Ariga Hiroyoshi; Tsutsumi Ken-ichi

Title	en Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene.
Creator	en Saitoh, Yasushi NRID 1000070287100 en Miyagi, Satoru en Ariga, Hiroyoshi NRID 1000020143505 en Tsutsumi, Ken-ichi
Accessrights	open access
Subject	MeSH en Amino Acid Sequence MeSH en Animals MeSH en Binding Sites MeSH en Cricetinae MeSH en DNA-Binding Proteins/chemistry MeSH en DNA-Binding Proteins/metabolism MeSH en Fructose-Bisphosphate Aldolase/genetics MeSH en Heterogeneous-Nuclear Ribonucleoprotein Group A-B MeSH en Humans MeSH en Mice MeSH en Molecular Sequence Data MeSH en Nuclear Proteins/chemistry MeSH en Nuclear Proteins/metabolism MeSH en Origin Recognition Complex MeSH en Promoter Regions, Genetic MeSH en Protein Structure, Tertiary MeSH en Rats MeSH en Repressor Proteins/chemistry MeSH en Repressor Proteins/metabolism MeSH en Sequence Alignment MeSH en Transcription, Genetic NDC 499
Description	Abstract en The promoter of the rat aldolase B (AldB) gene functions in vivo as an origin of DNA replication in the cells in which transcription of the gene is repressed. Previously, we identified two closely related DNA-binding proteins, AlF-C1 and AlF-C2, which repressed the AldB gene promoter. We also reported that the binding site of these proteins, site C, is one of the required DNA elements of the AldB gene origin/promoter for autonomously replicating activity in transfected cells. In the present study, we show that AlF-C1 and AlF-C2 bind directly to Orc1, a subunit of the origin recognition complex (ORC). Deletion analyses revealed a functional domain in AlF-C2 for binding to Orc1, which is located separately from the DNA-binding domain. In addition, we found a novel protein-interacting domain in Orc1 required for the binding of AlF-C2, which was conserved in human, mouse and Chinese hamster, but not in Drosophila, frog and yeast. Thus, it is assumed that in mammalian cells, sequence- specific DNA-binding proteins are involved in recruiting ORC to regulate replication initiation and/or transcription repression.
Publisher	en Oxford University Press
Date	Issued2002-12-01
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/53742
Relation	isIdenticalTo DOI https://doi.org/10.1093/nar/gkf642 PMID 12466545
Journal	PISSN 1362-4962 en Nucleic acids research Volume Number30 Issue Number23 Page Start5205 Page End5212
File	fulltext 127_Ariga_2002_Nucleic_Acids_Res.pdf 206.4 KB (application/pdf) Issued2002-12-01
Oaidate	2023-07-26