| Title |
-
en
Determination of the functional domain of a mouse autonomous replicating sequence.
|
| Creator |
|
| Accessrights |
open access |
| Subject |
-
Other
en
ARS
-
Other
en
c-myc
-
Other
en
replication
-
MeSH
en
Animals
-
MeSH
en
Anti-Bacterial Agents/pharmacology
-
MeSH
en
Base Sequence
-
MeSH
en
Cell Line
-
MeSH
en
Cloning, Molecular
-
MeSH
en
DNA Replication/genetics
-
MeSH
en
DNA, Recombinant/biosynthesis
-
MeSH
en
Drug Resistance/genetics
-
MeSH
en
Hygromycin B/pharmacology
-
MeSH
en
Mice
-
MeSH
en
Molecular Sequence Data
-
MeSH
en
Plasmids
-
MeSH
en
Rats
-
MeSH
en
Regulatory Sequences, Nucleic Acid/genetics
-
MeSH
en
Transfection
-
NDC
499
|
| Description |
-
Abstract
en
We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7, 1-6, 1987). Here we report the nucleotide sequence of ARS65. The sequence from BgIII to EcoRI sites cloned as ARS was 2658 bp long. There exist three interesting domains: a TA repeat, a myc like box (essential sequence for c-myc ARS), and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hygromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65.
|
| Publisher |
en
The Pharmaceutical Society of Japan
|
| Date |
|
| Language |
|
| Resource Type |
journal article |
| Version Type |
VoR |
| Identifier |
HDL
http://hdl.handle.net/2115/53973
|
| Relation |
-
isIdenticalTo
DOI
https://doi.org/10.1248/bpb.20.690
-
PMID
9212992
|
| Journal |
-
-
en
Biological & pharmaceutical bulletin
-
Volume Number20
Issue Number6
Page Start690
Page End693
|
| File |
|
| Oaidate |
2023-07-26 |
