Molecular cloning of MSSP-2, a c-myc gene single-strand binding protein: characterization of binding specificity and DNA replication activity.

Takai Toshiki; Nishita Yoshinori; Iguchi-Ariga Sanae M. M.; Ariga Hiroyoshi

Title	en Molecular cloning of MSSP-2, a c-myc gene single-strand binding protein: characterization of binding specificity and DNA replication activity.
Creator	en Takai, Toshiki en Nishita, Yoshinori en Iguchi-Ariga, Sanae M. M. NRID 1000090184283 en Ariga, Hiroyoshi NRID 1000020143505
Accessrights	open access
Subject	MeSH en Base Sequence MeSH en Cloning, Molecular MeSH en Consensus Sequence MeSH en DNA Primers/chemistry MeSH en DNA Replication MeSH en DNA, Complementary/genetics MeSH en DNA-Binding Proteins/genetics MeSH en DNA-Binding Proteins/metabolism MeSH en Genes, myc MeSH en Humans MeSH en Molecular Sequence Data MeSH en Mutagenesis, Site-Directed MeSH en RNA-Binding Proteins MeSH en Sequence Alignment MeSH en Sequence Homology, Amino Acid MeSH en Structure-Activity Relationship NDC 499
Description	Abstract en We have previously reported the human cDNA encoding MSSP-1, a sequence-specific double- and single-stranded DNA binding protein [Negishi, Nishita, Saëgusa, Kakizaki, Galli, Kihara, Tamai, Miyajima, Iguchi-Ariga and Ariga (1994) Oncogene, 9, 1133-1143]. MSSP-1 binds to a DNA replication origin/transcriptional enhancer of the human c-myc gene and has turned out to be identical with Scr2, a human protein which complements the defect of cdc2 kinase in S.pombe [Kataoka and Nojima (1994) Nucleic Acid Res., 22, 2687-2693]. We have cloned the cDNA for MSSP-2, another member of the MSSP family of proteins. The MSSP-2 cDNA shares highly homologous sequences with MSSP-1 cDNA, except for the insertion of 48 bp coding 16 amino acids near the C-terminus. Like MSSP-1, MSSP-2 has RNP-1 consensus sequences. The results of the experiments using bacterially expressed MSSP-2, and its deletion mutants, as histidine fusion proteins suggested that the binding specificity of MSSP-2 to double- and single-stranded DNA is the same as that of MSSP-1, and that the RNP consensus sequences are required for the DNA binding of the protein. MSSP-2 stimulated the DNA replication of an SV40-derived plasmid containing the binding sequence for MSSP-1 or -2. MSSP-2 is hence suggested to play an important role in regulation of DNA replication.
Publisher	en Oxford University Press
Date	Issued1994-12-25
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/54041
Relation	isIdenticalTo DOI https://doi.org/10.1093/nar/22.25.5576 PMID 7838710
Journal	PISSN 0305-1048 en Nucleic acids research Volume Number22 Issue Number25 Page Start5576 Page End5581
File	fulltext 68_Ariga_1994_Nucleic_Acids_Res.pdf 1.64 MB (application/pdf) Issued1994-12-25
Oaidate	2023-07-26