c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.

Iguchi-Ariga Sanae M. M.; Itani Teru; Yamaguchi Masamitsu; Ariga Hiroyoshi

Title	en c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
Creator	en Iguchi-Ariga, Sanae M. M. NRID 1000090184283 en Itani, Teru en Yamaguchi, Masamitsu en Ariga, Hiroyoshi NRID 1000020143505
Accessrights	open access
Subject	MeSH en Animals MeSH en Antibodies, Monoclonal/immunology MeSH en Antigens, Polyomavirus Transforming MeSH en Antigens, Viral, Tumor/physiology MeSH en Cell Line MeSH en Cercopithecus aethiops MeSH en DNA Replication/drug effects MeSH en DNA, Recombinant/biosynthesis MeSH en DNA, Viral/biosynthesis MeSH en DNA, Viral/genetics MeSH en DNA-Binding Proteins/pharmacology MeSH en Humans MeSH en Oncogene Proteins, Viral/physiology MeSH en Plasmids MeSH en Proto-Oncogene Proteins/immunology MeSH en Proto-Oncogene Proteins/pharmacology MeSH en Proto-Oncogene Proteins c-myc MeSH en Recombinant Proteins/pharmacology MeSH en Simian virus 40/genetics MeSH en Transfection NDC 499
Description	Abstract en Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
Publisher	en Oxford University Press
Date	Issued1987-06-25
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/54049
Relation	isIdenticalTo DOI https://doi.org/10.1093/nar/15.12.4889 PMID 3037484
Journal	PISSN 0305-1048 en Nucleic acids research Volume Number15 Issue Number12 Page Start4889 Page End4899
File	fulltext 27_Ariga_1987_Nucleic_Acids_Res.pdf 1.12 MB (application/pdf) Issued1987-06-25
Oaidate	2023-07-26