| Title |
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en
Adenovirus DNA synthesized in the presence of aphidicolin.
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| Creator |
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| Accessrights |
open access |
| Subject |
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MeSH
en
Adenoviruses, Human/drug effects
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MeSH
en
Adenoviruses, Human/genetics
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MeSH
en
Antiviral Agents/pharmacology
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MeSH
en
Aphidicolin
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MeSH
en
Carcinoma
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MeSH
en
Cell Line
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MeSH
en
Cell Transformation, Viral
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MeSH
en
DNA Replication/drug effects
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MeSH
en
DNA, Viral/genetics
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MeSH
en
DNA, Viral/isolation & purification
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MeSH
en
Diterpenes/pharmacology
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MeSH
en
Humans
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MeSH
en
Kinetics
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MeSH
en
Mouth Neoplasms
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NDC
499
|
| Description |
-
Abstract
en
Adenovirus types 2 and 5 DNA synthesized in vivo and in vitro in the presence of aphidicolin were studied. Inhibition of adenoviral DNA synthesis by aphidicolin was only 70% even at a concentration of 30 micrograms/ml of aphidicolin, at which the cellular DNA synthesis was completely inhibited. When initiation of the viral DNA synthesis was synchronized with hydroxyurea and labeled with [3H]thymidine for 60 min, the viral DNA synthesized in the presence of 30 micrograms/ml of aphidicolin was not of full length (35 kb) but small (approximately 12 kb) by analysis of alkaline sucrose density gradient centrifugation. When initiation of the viral DNA synthesis was not synchronized, the viral DNAs ranging from full size to 12 kb were synthesized in the presence of aphidicolin, indicating that the nascent DNAs longer than about 12 kb can continue to elongate in the presence of aphidicolin. This 12 kb DNA was not derived from the degradation products of newly synthesized full size adenoviral DNA. The viral DNA synthesis was restored and the full size of adenoviral DNA was attained within 15 min following removal of aphidicolin. About 20% of the entire viral genome length from the 5'-end was not inhibited by aphidicolin, while the synthesis of interior fragments of the adenoviral DNA was markedly inhibited by aphidicolin, judging from the electrophoretic pattern on neutral agarose gel after digestion of DNA with Hind III. These results indicate that aphidicolin inhibits adenoviral DNA replication at the internal region located approximately 20-30% from both terminals.
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| Publisher |
en
Oxford University Press
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
VoR |
| Identifier |
HDL
http://hdl.handle.net/2115/54052
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| Relation |
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isIdenticalTo
DOI
https://doi.org/10.1093/nar/12.2.1077
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PMID
6420772
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| Journal |
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en
Nucleic acids research
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Volume Number12
Issue Number2
Page Start1077
Page End1086
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| File |
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| Oaidate |
2023-07-26 |
