Polymerase complex with lysine at position 627 of the PB2 of influenza virus A/Hong Kong/483/97 (H5N1) efficiently transcribes and replicates virus genes in mouse cells

Yamamoto Naoki; Sakoda Yoshihiro; Okamatsu Masatoshi; Kida Hiroshi

Title	en Polymerase complex with lysine at position 627 of the PB2 of influenza virus A/Hong Kong/483/97 (H5N1) efficiently transcribes and replicates virus genes in mouse cells
Creator	en Yamamoto, Naoki en Sakoda, Yoshihiro NRID 1000040333637 en Okamatsu, Masatoshi NRID 1000000507163 en Kida, Hiroshi NRID 1000010109506
Accessrights	open access
Subject	Other en PB2 Other en H5N1 Other en Influenza A virus Other en Polymerase NDC 649
Description	Abstract en Influenza virus A/Hong Kong/483/97 (H5N1) (HK483-K) has the PB2 with lysine at position 627 (PB2-627K) and is highly pathogenic in chickens and mice. On the other hand, the pathogenicity of mutant virus (HK483-E), which was generated by substituting lysine with glutamic acid at the position of the PB2, is lower than that of HK483-K in mice, but is highly pathogenic in chickens. The PB2 is one of the components of heterotrimeric polymerase complex, which plays roles in the transcription and replication of virus genes. Cell-free polymerase assay revealed that intrinsic transcription activity of the polymerase complex with PB2-627K is higher than that of glutamic acid (PB2-627E). In chicken cells, transcription efficiency of the polymerase complex with PB2-627E was not lower than those with PB2-627K, indicating that transcription of virus genes is modulated by some host factors in chicken cells, resulting in high growth. Polymerase complex with PB2-627K efficiently transcribes and replicates virus polymerase genes in mouse cells, leading to high growth of HK483-K compared with that of HK483-E. The results of our experiments clearly suggest that efficient transcription and replication of virus genes by polymerase complex result in the higher pathogenicity in mice. (C) 2013 Elsevier B.V. All rights reserved.
Publisher	en Elsevier
Date	Issued2013-12-26
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/54759
Relation	isVersionOf DOI https://doi.org/10.1016/j.virusres.2013.09.012 PMID 24070983
Journal	PISSN 0168-1702 en Virus Research Volume Number178 Issue Number2 Page Start404 Page End410
File	fulltext VR revised manuscript (Yamamoto130731).pdf 461.49 KB (application/pdf) Issued2013-12-26
Oaidate	2023-07-26