| Title |
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The effect of copper on the mRNA expression profile of xenobiotic-metabolizing enzymes in cultured rat H4-II-E cells
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| Creator |
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| Accessrights |
open access |
| Rights |
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en
The final publication is available at www.springerlink.com
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| Subject |
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Other
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Copper
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Other
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Hepatocyte
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Other
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Cytochrome P450
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Other
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Phase II enzymes
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Other
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Aryl hydrocarbon receptor
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NDC
649
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| Description |
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Abstract
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Copper (Cu2+) is an essential element that plays important roles in physiological functions of the body. However, high Cu2+ levels can have toxic implications. This study aims to investigate the constitutive response to Cu2+ exposure of xenobiotic-metabolizing enzymes in cultured rat liver (H4-II-E) cell lines. Rat cells were exposed to copper sulfate (0-500 mu M) for 24 h. The effects of Cu2+ on the messenger RNA (mRNA) expressions of phase I and II enzymes and regulatory elements were examined using real-time PCR. Metallothionein mRNA expression was induced in a dose-dependent manner after treatment with Cu2+. mRNA expressions of phase I enzymes such as cytochrome P450 1A1 and 1A2 (CYP1A1 and CYP1A2) were slightly induced after exposure to low concentrations of Cu2+; however, CYP1A1 and CYP1A2 mRNA expressions were significantly downregulated at higher Cu2+ concentrations. These effects corresponded with expression of aryl hydrocarbon receptor mRNA. The mRNA expressions of phase II enzymes were reduced upon exposure to Cu2+. In conclusion, phase I and II enzyme expressions were significantly modulated upon Cu2+ exposure. These results indicated that Cu2+ exposure had toxicological implications for cultured H4-II-E cells.
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| Publisher |
en
Springer
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| Date |
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| Language |
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| Resource Type |
journal article |
| Version Type |
AM |
| Identifier |
HDL
http://hdl.handle.net/2115/58553
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| Relation |
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isVersionOf
DOI
https://doi.org/10.1007/s12011-014-9915-9
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PMID
24599699
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| Journal |
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PISSN
0163-4984
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NCID
AA00110004
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en
Biological Trace Element Research
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Volume Number158
Issue Number2
Page Start243
Page End248
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| File |
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| Oaidate |
2023-07-26 |
