Title |
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Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus
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Creator |
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Accessrights |
open access |
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Description |
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Abstract
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Multiple host molecules are known to be involved in the cellular entry of filoviruses, including Ebola virus (EBOV); T-cell immunoglobulin and mucin domain 1 (TIM-1) and Niemann-Pick C1 (NPC1) have been identified as attachment and fusion receptors, respectively. However, the molecular mechanisms underlying the entry process have not been fully understood. We found that TIM-1 and NPC1 colocalized and interacted in the intracellular vesicles where EBOV glycoprotein (GP)-mediated membrane fusion occurred. Interestingly, a TIM-1-specific monoclonal antibody (MAb), M224/1, prevented GP-mediated membrane fusion and also interfered with the binding of TIM-1 to NPC1, suggesting that the interaction between TIM-1 and NPC1 is important for filovirus membrane fusion. Moreover, MAb M224/1 efficiently inhibited the cellular entry of viruses from all known filovirus species. These data suggest a novel mechanism underlying filovirus membrane fusion and provide a potential cellular target for antiviral compounds that can be universally used against filovirus infections.
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Publisher |
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American Society for Microbiology
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Date |
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Language |
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Resource Type |
journal article |
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VoR |
Identifier |
HDL
http://hdl.handle.net/2115/59504
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Relation |
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isIdenticalTo
DOI
https://doi.org/10.1128/JVI.03156-14
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Journal |
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PISSN
0022-538X
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EISSN
1098-5514
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NCID
AA00708779
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Journal of virology
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Volume Number89
Issue Number12
Page Start6481
Page End6493
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File |
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Oaidate |
2023-07-26 |