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Title
  • en Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus
Creator
    • en Kuroda, Makoto
    • en Marzi, Andrea
    • en Noyori, Osamu
    • en Kajihara, Masahiro
    • en Maruyama, Junki
    • en Matsuno, Keita
    • en Miyamoto, Hiroko
    • en Yoshida, Reiko
    • en Feldmann, Heinz
Accessrights open access
Subject
  • NDC 493
Description
  • Abstract en Multiple host molecules are known to be involved in the cellular entry of filoviruses, including Ebola virus (EBOV); T-cell immunoglobulin and mucin domain 1 (TIM-1) and Niemann-Pick C1 (NPC1) have been identified as attachment and fusion receptors, respectively. However, the molecular mechanisms underlying the entry process have not been fully understood. We found that TIM-1 and NPC1 colocalized and interacted in the intracellular vesicles where EBOV glycoprotein (GP)-mediated membrane fusion occurred. Interestingly, a TIM-1-specific monoclonal antibody (MAb), M224/1, prevented GP-mediated membrane fusion and also interfered with the binding of TIM-1 to NPC1, suggesting that the interaction between TIM-1 and NPC1 is important for filovirus membrane fusion. Moreover, MAb M224/1 efficiently inhibited the cellular entry of viruses from all known filovirus species. These data suggest a novel mechanism underlying filovirus membrane fusion and provide a potential cellular target for antiviral compounds that can be universally used against filovirus infections.
Publisher en American Society for Microbiology
Date
    Issued2015-06
Language
  • eng
Resource Type journal article
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/59504
Relation
  • isIdenticalTo DOI https://doi.org/10.1128/JVI.03156-14
Journal
    • PISSN 0022-538X
    • EISSN 1098-5514
    • NCID AA00708779
      • en Journal of virology
      • Volume Number89 Issue Number12 Page Start6481 Page End6493
File
Oaidate 2023-07-26