Title |
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RANKL regulates differentiation of microfold cells in mouse nasopharynx-associated lymphoid tissue (NALT)
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Creator |
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Accessrights |
open access |
Rights |
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en
The final publication is available at link.springer.com
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Subject |
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Other
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Microfold cells (M cells)
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Other
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Follicle-associated epithelium
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Other
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Nasopharynx-associated lymphoid tissue (NALT)
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Other
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Glycoprotein 2 (GP2)
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Other
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Receptor activator of nuclear factor kappa-B ligand (RANKL)
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NDC
490
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Description |
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Abstract
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Murine nasopharynx-associated lymphoid tissue (NALT), located at the base of the nasal cavity, serves as a major site for the induction of mucosal immune responses against airway antigens. The follicle-associated epithelium (FAE) covering the luminal surface of NALT is characterized by the presence of microfold cells (M cells), which take up and transport luminal antigens to lymphocytes. Glycoprotein 2 (GP2) has recently been identified as a reliable marker for M cells in Peyer's patches of the intestine. However, the expression of GP2 and other functional molecules in the M cells of NALT has not yet been examined. We have immunohistochemically detected GP2-expressing cells in the FAE of NALT and the simultaneous expression of other intestinal M-cell markers, namely Tnfaip2, CCL9, and Spi-B. These cells have been further identified as M cells because of their higher uptake capacity of luminal microbeads. Electron microscopic observations have shown that GP2-expressing cells on the FAE display morphological features typical of M cells: they possess short microvilli and microfolds on the luminal surface and are closely associated with intraepithelial lymphocytes. We have also found that the receptor activator of nuclear factor kappa-B ligand (RANKL) is expressed by stromal cells underneath the FAE, which provides its receptor RANK. The administration of RANKL markedly increases the number of GP2+Tnfaip2+ cells on the NALT FAE and that of intestinal M cells. These results suggest that GP2+Tnfaip2+ cells in NALT are equivalent to intestinal M cells, and that RANKL-RANK signaling induces their differentiation.
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Publisher |
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Springer
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Date |
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Language |
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Resource Type |
journal article |
Version Type |
AM |
Identifier |
HDL
http://hdl.handle.net/2115/64947
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Relation |
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isVersionOf
DOI
https://doi.org/10.1007/s00441-015-2309-2
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PMID
26553655
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Journal |
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PISSN
0302-766X
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EISSN
1432-0878
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en
Cell and Tissue Research
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Volume Number364
Issue Number1
Page Start175
Page End184
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File |
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Oaidate |
2023-07-26 |