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Title
  • en RANKL regulates differentiation of microfold cells in mouse nasopharynx-associated lymphoid tissue (NALT)
Creator
Accessrights open access
Rights
  • en The final publication is available at link.springer.com
Subject
  • Other en Microfold cells (M cells)
  • Other en Follicle-associated epithelium
  • Other en Nasopharynx-associated lymphoid tissue (NALT)
  • Other en Glycoprotein 2 (GP2)
  • Other en Receptor activator of nuclear factor kappa-B ligand (RANKL)
  • NDC 490
Description
  • Abstract en Murine nasopharynx-associated lymphoid tissue (NALT), located at the base of the nasal cavity, serves as a major site for the induction of mucosal immune responses against airway antigens. The follicle-associated epithelium (FAE) covering the luminal surface of NALT is characterized by the presence of microfold cells (M cells), which take up and transport luminal antigens to lymphocytes. Glycoprotein 2 (GP2) has recently been identified as a reliable marker for M cells in Peyer's patches of the intestine. However, the expression of GP2 and other functional molecules in the M cells of NALT has not yet been examined. We have immunohistochemically detected GP2-expressing cells in the FAE of NALT and the simultaneous expression of other intestinal M-cell markers, namely Tnfaip2, CCL9, and Spi-B. These cells have been further identified as M cells because of their higher uptake capacity of luminal microbeads. Electron microscopic observations have shown that GP2-expressing cells on the FAE display morphological features typical of M cells: they possess short microvilli and microfolds on the luminal surface and are closely associated with intraepithelial lymphocytes. We have also found that the receptor activator of nuclear factor kappa-B ligand (RANKL) is expressed by stromal cells underneath the FAE, which provides its receptor RANK. The administration of RANKL markedly increases the number of GP2+Tnfaip2+ cells on the NALT FAE and that of intestinal M cells. These results suggest that GP2+Tnfaip2+ cells in NALT are equivalent to intestinal M cells, and that RANKL-RANK signaling induces their differentiation.
Publisher en Springer
Date
    Issued2016-04
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/64947
Relation
  • isVersionOf DOI https://doi.org/10.1007/s00441-015-2309-2
  • PMID 26553655
Journal
    • PISSN 0302-766X
    • EISSN 1432-0878
      • en Cell and Tissue Research
      • Volume Number364 Issue Number1 Page Start175 Page End184
File
Oaidate 2023-07-26