Receptor activator of NF-κB ligand induces cell adhesion and integrin α2 expression via NF-κB in head and neck cancers

Yamada Tamaki; Tsuda Masumi; Wagatsuma Takanori; Fujioka Yoichiro; Fujioka Mari; Satoh Aya O.; Horiuchi Kosui; Nishide Shinya; Nanbo Asuka; Totsuka Yasunori; Haga Hisashi; Tanaka Shinya; Shindoh Masanobu; Ohba Yusuke

Title	en Receptor activator of NF-κB ligand induces cell adhesion and integrin α2 expression via NF-κB in head and neck cancers
Creator	en Yamada, Tamaki NRID 1000080580943 en Tsuda, Masumi NRID 1000030431307 en Wagatsuma, Takanori en Fujioka, Yoichiro en Fujioka, Mari en Satoh, Aya O. en Horiuchi, Kosui en Nishide, Shinya NRID 1000040451398 en Nanbo, Asuka NRID 1000060359487 en Totsuka, Yasunori NRID 1000000109456 en Haga, Hisashi NRID 1000000292045 en Tanaka, Shinya NRID 1000070261287 en Shindoh, Masanobu NRID 1000020162802 en Ohba, Yusuke NRID 1000030333503
Accessrights	open access
Rights	http://creativecommons.org/licenses/by/4.0/ en Creative Commons Attribution 4.0 International
Subject	NDC 490
Description	Abstract en Cellular interactions with the extracellular matrix play critical roles in tumor progression. We previously reported that receptor activator of NF-κB ligand (RANKL) specifically facilitates head and neck squamous cell carcinoma (HNSCC) progression in vivo. Here, we report a novel role for RANKL in the regulation of cell adhesion. Among the major type I collagen receptors, integrin α2 was significantly upregulated in RANKL-expressing cells, and its knockdown suppressed cell adhesion. The mRNA abundance of integrin α2 positively correlated with that of RANKL in human HNSCC tissues. We also revealed that RANK-NF-κB signaling mediated integrin α2 expression in an autocrine/paracrine manner. Interestingly, the amount of active integrin β1 on the cell surface was increased in RANKL-expressing cells through the upregulation of integrin α2 and endocytosis. Moreover, the RANK-integrin α2 pathway contributed to RANKL-dependent enhanced survival in a collagen gel and inhibited apoptosis in a xenograft model, demonstrating an important role for RANKL-mediated cell adhesion in three-dimensional environments.
Publisher	en Nature Publishing Group
Date	Issued2016-03-24
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/61496
Relation	isIdenticalTo DOI https://doi.org/10.1038/srep23545
Journal	PISSN 2045-2322 en Scientific reports Volume Number6 Page Start23545
File	fulltext srep23545-s1.mov 1.31 MB (video/quicktime) Issued2016-03-24 fulltext srep23545-s2.mov 3.31 MB (video/quicktime) Issued2016-03-24 fulltext srep23545.pdf 4.61 MB (application/pdf) Issued2016-03-24
Oaidate	2023-07-26