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Title	en Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation
Creator	en Kimura, Shunsuke NRID 1000040444525 en Yamashita, Masami en Yamakami-Kimura, Megumi en Sato, Yusuke en Yamagata, Atsushi NRID 1000020463903 en Kobashigawa, Yoshihiro en Inagaki, Fuyuhiko NRID 1000070011757 en Amada, Takako en Hase, Koji NRID 1000020359714 en Iwanaga, Toshihiko NRID 1000010160128 en Ohno, Hiroshi NRID 1000050233226 en Fukai, Shuya NRID 1000010361792
Accessrights	open access
Rights	https://creativecommons.org/licenses/by/4.0/ en Creative Commons Attribution 4.0 International
Subject	NDC 490
Description	Abstract en The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.
Publisher	en Nature Publishing Group
Date	Issued2016-09-15
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/63450
Relation	isIdenticalTo DOI https://doi.org/10.1038/srep33548
Journal	PISSN 2045-2322 en Scientific reports Volume Number6 Page Start33548
File	fulltext srep33548.pdf 1.64 MB (application/pdf) Issued2016-09-15
Oaidate	2023-07-26