Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis

Saeki Ayumi; Suzuki Toshihiko; Hasebe Akira; Kamezaki Ryousuke; Fujita Mari; Nakazawa Futoshi; Shibata Ken-Ichiro

Title	en Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis
Creator	en Saeki, Ayumi NRID 1000070638345 en Suzuki, Toshihiko en Hasebe, Akira NRID 1000090281815 en Kamezaki, Ryousuke en Fujita, Mari en Nakazawa, Futoshi en Shibata, Ken-Ichiro NRID 1000050145265
Accessrights	open access
Rights	en This is the peer reviewed version of the following article: [Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis.], which has been published in final form at [http://doi.org/10.1111/cmi.12663]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Subject	NDC 497
Description	Abstract en Streptococcus sanguinis is frequently isolated from the blood of patients with infective endocarditis and contributes to the pathology of this disease through induction of interleukin (IL)-1β responsible for the development of the disease. However, the mechanism of IL-1β induction remains unknown. In this study, S. sanguinis activated a murine dendritic cell (DC) to induce IL-1β and this activity was attenuated by silencing the mRNAs of nucleotide-binding domain-like receptor containing protein 3 (NLRP3) and caspase-1. S. sanguinis induced IL-1β production in murine bone marrow-derived macrophage (BMM), but this activity was significantly reduced in BMMs from NLRP3-, apoptosis-associated speck-like protein containing a caspase-recruitment domain-, and caspase-1-deficient mice. DC phagocytosed S. sanguinis cells, followed by the release of adenosine triphosphate (ATP). The ATP-degradating enzyme attenuated the release of ATP and IL-1β. The inhibitors for ATP receptor reduced IL-1β release in DC. These results strongly suggest that S. sanguinis has the activity to induce IL-1β through the NLRP3 inflammasome in macrophage and DC and interaction of purinergic receptors with ATP released is involved in expression of the activity.
Publisher	en Wiley-Blackwell
Date	Issued2017-03
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/68426
Relation	isVersionOf DOI https://doi.org/10.1111/cmi.12663 PMID 27601185
Journal	PISSN 1462-5814 EISSN 1462-5822 en Cellular Microbiology Volume Number19 Issue Number3 Page Starte12663
File	fulltext CM S sanguinis 2nd revised.pdf 873.8 KB (application/pdf) Issued2017-03
Oaidate	2023-08-19