Title |
-
en
Stereospecific Analysis of Fish Oil Triacylglycerols by Silicic Acid HPLC Using Monoacylglycerol Analytical Intermediates
|
Creator |
|
Accessrights |
open access |
Subject |
-
Other
en
Fish oil
-
Other
en
Triacylglycerol
-
Other
en
Stereospecific analysis
-
Other
en
HPLC
-
Other
en
1-(1-Naphthyl)ethyl urethane
-
NDC
660
|
Description |
-
Abstract
en
This study establishes a method for the stereospecific analysis of fish oil triacylglycerols (TAG) using silicic acid high-performance liquid chromatography (HPLC). The analysis includes partial degradation of TAG to monoacylglycerols (MAG), derivatization of MAG to (R)-(−)-1-(1-naphthyl)ethyl urethanes, fractionation of sn-1-, sn-2-, and sn-3-MAG by HPLC, and fatty acid analysis of methyl esters by gas chromatography. For the analysis of fish oil TAG, several modifications were required in the original method developed for the analysis of olive oil TAG. For the HPLC analysis, reducing the proportion of 2-propanol in the mobile phase enabled clear separation of sn-1- and sn-3-MAG, which consisted of a wide variety of fatty acids of fish origin. Another improvement that was made was purifying the fatty acid methyl esters prepared from MAG urethanes by thin-layer chromatography. Stereospecific analysis was achieved when commencing with only 1 mg of fish oil TAG. Acyl migration, which affects the accuracy of the analysis, was found to be less than 5%. Using this method, stereospecific analysis can be carried out by HPLC on a conventional silica column, in contrast to the conventional HPLC method that requires expensive chiral columns. This method can also be used for the analysis of the TAG of both common and rare fish.
|
Publisher |
ja
北海道大学大学院水産科学研究院
|
Date |
|
Language |
|
Resource Type |
departmental bulletin paper |
Version Type |
VoR |
Identifier |
DOI
https://doi.org/10.14943/bull.fish.68.2.33
,
HDL
http://hdl.handle.net/2115/71151
|
ID |
-
JaLC
10.14943/bull.fish.68.2.33
|
Journal |
-
-
PISSN
1346-1842
-
NCID
AA11547977
-
ja
北海道大学水産科学研究彙報
-
Volume Number68
Issue Number2
Page Start33
Page End41
|
File |
|
Oaidate |
2023-07-26 |