Back

Title
  • en Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
Creator
    • en Toraya, Hisashi
    • en Sakagami, Naoko
    • en Tsuchiya, Erika
    • en Kudo, Ai
    • en Zhao, Shen
    • en Moritani, Yasuhito
    • en Abe, Miki
    • en Yoshida, Taiji
    • en Yamamoto, Tomomaya
    • en Udagawa, Nobuyuki
    • en Freitas, PHL.
Accessrights open access
Subject
  • NDC 497
Description
  • Abstract en Since osteoblastic activities are believed to be coupled with osteoclasts, we have attempted to histologically verify which of the distinct cellular circumstances, the presence of osteoclasts themselves or bone resorption by osteoclasts, is essential for coupled osteoblastic activity, by examining c-fos−/− or c-src−/− mice. Osteopetrotic c-fos deficient (c-fos−/−) mice have no osteoclasts, while c-src deficient (c-src−/−) mice, another osteopetrotic model, develop dysfunctional osteoclasts due to a lack of ruffled borders. c-fos−/− mice possessed no tartrate-resistant acid phosphatase (TRAPase)-reactive osteoclasts, and showed very weak tissue nonspecific alkaline phosphatase (TNALPase)-reactive mature osteoblasts. In contrast, c-src−/− mice had many TNALPase-positive osteoblasts and TRAPase-reactive osteoclasts. Interestingly, the parallel layers of TRAPase-reactive/osteopontin-positive cement lines were observed in the superficial region of c-src−/− bone matrix. This indicates the possibility that in c-src−/− mice, osteoblasts were activated to deposit new bone matrices on the surfaces that osteoclasts previously passed along, even without bone resorption. Transmission electron microscopy demonstrated cell-to-cell contacts between mature osteoblasts and neighboring ruffled border-less osteoclasts, and osteoid including many mineralized nodules in c-src−/− mice. Thus, it seems likely that osteoblastic activities would be maintained in the presence of osteoclasts, even if they are dysfunctional.
Publisher en Biomedical Research Press
Date
    Issued2017-04-01
Language
  • eng
Resource Type journal article
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/80591
Relation
  • isIdenticalTo DOI https://doi.org/10.2220/biomedres.38.123
Journal
    • PISSN 0388-6107
    • EISSN 1880-313X
    • NCID AA00110128
      • en Biomedical Research
      • Volume Number38 Issue Number2 Page Start123 Page End134
File
Oaidate 2023-07-26