| タイトル |
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en
Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens
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| 作成者 |
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| アクセス権 |
open access |
| 権利情報 |
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en
The original publication is available at www.springerlink.com
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| 主題 |
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Other
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androgenic callus
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Other
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anther culture
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Other
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dihaploid
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Other
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GUS activity
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Other
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transgenic forage grass
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NDC
616
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| 内容注記 |
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Abstract
en
Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgenes was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n=2x=14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.
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| 出版者 |
en
Springer
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/42593
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| 関連 |
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isVersionOf
DOI
https://doi.org/10.1007/s11240-008-9478-6
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| 収録誌情報 |
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PISSN
0167-6857
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EISSN
1573-5044
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NCID
AA10472996
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en
Plant Cell, Tissue and Organ Culture
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巻96
号2
開始ページ219
終了ページ227
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
