| Title |
-
en
Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens
|
| Creator |
|
| Accessrights |
open access |
| Rights |
-
en
The original publication is available at www.springerlink.com
|
| Subject |
-
Other
en
androgenic callus
-
Other
en
anther culture
-
Other
en
dihaploid
-
Other
en
GUS activity
-
Other
en
transgenic forage grass
-
NDC
616
|
| Description |
-
Abstract
en
Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgenes was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n=2x=14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.
|
| Publisher |
en
Springer
|
| Date |
|
| Language |
|
| Resource Type |
journal article |
| Version Type |
AM |
| Identifier |
HDL
http://hdl.handle.net/2115/42593
|
| Relation |
-
isVersionOf
DOI
https://doi.org/10.1007/s11240-008-9478-6
|
| Journal |
-
-
PISSN
0167-6857
-
EISSN
1573-5044
-
NCID
AA10472996
-
en
Plant Cell, Tissue and Organ Culture
-
Volume Number96
Issue Number2
Page Start219
Page End227
|
| File |
|
| Oaidate |
2023-07-26 |
