| タイトル |
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ja
加熱損傷ウェルシュ菌の検出培地の比較と改良
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en
Comparison and Improvement of Detection Media for Sub-lethal Injured Clostridium perfringens
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| 作成者 |
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ja
清水, 茂雅
en
Shimizu, Shigemasa
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ja
堀口, 明日香
en
Horiguchi, Asuka
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| アクセス権 |
open access |
| 主題 |
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Other
en
Clostridium perfringens
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Other
en
Injured cell
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Other
en
Recovery
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Other
en
Sodium pyruvate
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NDC
660
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| 内容注記 |
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Abstract
en
Recovery of heat-injured vegetative cells and spores of Clostridium perfringens was evaluated on selective media, CW Egg-yolk agar with kanamycin (ECW+A), Tryptose sulfite cycloserine agar(TSC+A)and modified Handford agar (mHFA). As a result of heat treatment at 54℃ for 15 min, viable counts of C. perfringens vegetative cells on a selective medium (ECW+A)was significantly less than those on non-selective media. This means C. perfringens vegetative cells should be in an injured state. Comparing of the three selective media, TSC+A was the best growth and recovery of heat-injured C. perfringens vegetative cells. Recovery on ECW+A was 1 log CFU/ml less than that on TSC+A. Supplementation of TSC+A with sodium pyruvate (0.1-0.5%) further enhanced recovery and detection of heat-injured C. perfringens vegetative cells, and its efficacy was the highest on TSC+A supplemented with 0.3% sodium pyruvate. Supplementation of TSC+A with sodium pyruvate did not affect recovery of heat-injured (95℃, 2 or 60 min) C. perfringens spores. These findings suggest that TSC+A is the most favorable medium for enumeration of C. perfringens and supplementation with sodium pyruvate improves recovery and detection of heat-injured C. perfringens.
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| 出版者 |
ja
北海道大学大学院水産科学研究院
en
Research Faculty of Fisheries Sciences, Hokkaido University
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| 日付 |
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| 言語 |
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| 資源タイプ |
departmental bulletin paper |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/39007
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| 収録誌情報 |
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ja
北海道大学水産科学研究彙報
en
Bulletin of Fisheries Sciences, Hokkaido University
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巻59
号2
開始ページ37
終了ページ42
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
