タイトル |
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en
Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood
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作成者 |
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アクセス権 |
open access |
主題 |
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Other
en
Bacteriocin
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Other
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Pediocin
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Other
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Pediococcus
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Other
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Medium components
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Other
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Culture condition
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Other
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Optimization
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NDC
660
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内容注記 |
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Abstract
en
Growth requirements for maximum production of pediocin Iz3.13 by Pediococcus pentosaceus Iz3.13 were studied. Physical and chemical parameters that enhanced pediocin production included incubation in static broth at 30℃, with an initial pH of 4.5. Optimum nutritional conditions that enhanced pediocin Iz3.13 production were 3.5% Trypticase peptone, 3.5% Phytone peptone or 5.0% Proteose peptone No.3 as nitrogen sources, and glucose or maltose as carbon sources. Supplementing growth media with beef extract and MgCl2 also enhanced production. This study proposes an optimized growth medium containing 5.0% Proteose peptone No.3, 3.5% Phytone peptone, 2.5% glucose, 1.5% MgCl2, 3.0% beef extract and 0.25% K2HPO4. This medium significantly enhanced pediocin Iz3.13 production (5.1 fold increase) as compared to a basal medium.
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出版者 |
ja
北海道大学大学院水産科学研究院
en
Research Faculty of Fisheries Sciences, Hokkaido University
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日付 |
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言語 |
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資源タイプ |
departmental bulletin paper |
出版タイプ |
VoR |
資源識別子 |
HDL
http://hdl.handle.net/2115/42764
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収録誌情報 |
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ja
北海道大学水産科学研究彙報
en
Bulletin of Fisheries Sciences, Hokkaido University
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巻60
号1
開始ページ5
終了ページ12
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ファイル |
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コンテンツ更新日時 |
2023-07-26 |