Temperature controls nuclear import of Tam3 transposase in Antirrhinum

Fujino Kaien; Hashida Shin-nosuke; Ogawa Takashi; Natsume Tomoko; Uchiyama Takako; Mikami Tetsuo; Kishima Yuji

タイトル	en Temperature controls nuclear import of Tam3 transposase in Antirrhinum
作成者	en Fujino, Kaien en Hashida, Shin-nosuke en Ogawa, Takashi en Natsume, Tomoko en Uchiyama, Takako en Mikami, Tetsuo en Kishima, Yuji NRID 1000060192556
アクセス権	open access
権利情報	en The definitive version is available at www.blackwell-synergy.com
主題	Other en low-temperature-dependent transposition (LTDT) Other en nuclear import Other en transposase (TPase) Other en transposon Tam3 Other en Antirrhinum majus NDC 471
内容注記	Abstract en It has been proposed that environmental stimuli can activate transposable elements (TEs), while few substantial mechanisms have been shown so far. The class II element Tam3 from Antirrhinum majus exhibits a unique property of low-temperature-dependent transposition (LTDT). LTDT has proved invaluable in developing the gene isolation technologies that have underpinned much of modern plant developmental biology. Here, we reveal that LTDT involves differential sub-cellular localization of the Tam3 transposase (TPase) in cells grown at low (15℃) and high (25℃) temperatures. The mechanism is associated with the nuclear import of Tam3 TPase in Antirrhinum cells. At high temperature, the nuclear import of Tam3 TPase is severely restricted in Antirrhinum cells, while at low temperature, the nuclear localization of Tam3 TPase is observed in about 20% of the cells. However, in tobacco BY-2 and onion cells, Tam3 TPase is transported into most nuclei. In addition to three nuclear localization signals (NLSs), the Tam3 TPase is equipped with a nuclear localization inhibitory domain (NLID), which functions to abolish nuclear import of the TPase at high temperature in Antirrhinum. NLID in Tam3 TPase is considered to interact with Antirrhinum specific factor(s). The host-specific regulation of the nuclear localization of transposase represents a new repertoire controlling class II TEs.
出版者	en Wiley-Blackwell
日付	Issued2011-01
言語	eng
資源タイプ	journal article
出版タイプ	AM
資源識別子	HDL http://hdl.handle.net/2115/47949
関連	isVersionOf DOI https://doi.org/10.1111/j.1365-313X.2010.04405.x PMID 21175897
収録誌情報	PISSN 0960-7412 en The Plant Journal 巻65 号1 開始ページ146 終了ページ155
ファイル	fulltext PJ65-1_146-155.pdf 32.45 MB (application/pdf) Issued2011-01 fulltext Supporting_Information_Figure_S1.pdf 21.63 KB (application/pdf) Issued2011-01 fulltext Supporting_Information_Figure_S2.pdf 835.35 KB (application/pdf) Issued2011-01 fulltext Supporting_Information_Legends.pdf 39.39 KB (application/pdf) Issued2011-01 fulltext Supporting_Information_Table_S1.pdf 19.12 KB (application/pdf) Issued2011-01 fulltext Supporting_Information_Table_S2.pdf 25.15 KB (application/pdf) Issued2011-01
コンテンツ更新日時	2023-07-26