| タイトル |
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en
Characterization of phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species : Unique pyrene metabolism and species specificity observed in fish species
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| 作成者 |
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| アクセス権 |
open access |
| 主題 |
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Other
en
Fish
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Other
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Pyrene
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Other
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Conjugation
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Other
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Polycyclic aromatic hydrocarbons (PAHs)
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Other
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Metabolism
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Other
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Inter-species difference
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NDC
649
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| 内容注記 |
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Abstract
en
Metabolic activity, particularly conjugation, was examined in fish by analyzing pyrene (a four-ring, polycyclic aromatic hydrocarbon) metabolites using high-performance liquid chromatography (HPLC) with fluorescence detector (FD), a mass spectrometry (MS) system, and kinetic analysis of conjugation enzymes. Fourteen fresh water fish species, including Danio rerio and Orizias latipes, were exposed to aqueous pyrene, and the resulting metabolites were collected. Identification of pyrene metabolites by HPLC/FD and ion-trap MS indicated that the major metabolites were pyrene glucuronide and pyrene sulfate in all 14 species. Differences were observed in pyrene glucuronide:pyrene sulfate ratio and in the total amount of pyrene conjugates excreted between fish species. Furthermore, a correlation was found between the amount of pyrene glucuronide present and the total amount of the pyrene metabolite eliminated. Kinetic analysis of conjugation by hepatic microsomes in vitro indicated that the differences in excreted metabolites reflected the differences in enzymatic activities. (C) 2013 Elsevier B.V. All rights reserved.
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| 出版者 |
en
Elsevier
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/53434
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| 関連 |
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isVersionOf
DOI
https://doi.org/10.1016/j.etap.2013.05.018
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PMID
23834960
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| 収録誌情報 |
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en
Environmental Toxicology and Pharmacology
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巻36
号2
開始ページ567
終了ページ578
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
