タイトル |
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Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene.
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作成者 |
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アクセス権 |
open access |
主題 |
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MeSH
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Amino Acid Sequence
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MeSH
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Animals
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MeSH
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Binding Sites
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MeSH
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Cricetinae
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MeSH
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DNA-Binding Proteins/chemistry
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MeSH
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DNA-Binding Proteins/metabolism
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MeSH
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Fructose-Bisphosphate Aldolase/genetics
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MeSH
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B
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MeSH
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Humans
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MeSH
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Mice
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MeSH
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Molecular Sequence Data
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MeSH
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Nuclear Proteins/chemistry
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MeSH
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Nuclear Proteins/metabolism
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MeSH
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Origin Recognition Complex
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MeSH
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Promoter Regions, Genetic
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MeSH
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Protein Structure, Tertiary
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MeSH
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Rats
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MeSH
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Repressor Proteins/chemistry
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MeSH
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Repressor Proteins/metabolism
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Sequence Alignment
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MeSH
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Transcription, Genetic
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NDC
499
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内容注記 |
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Abstract
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The promoter of the rat aldolase B (AldB) gene functions in vivo as an origin of DNA replication in the cells in which transcription of the gene is repressed. Previously, we identified two closely related DNA-binding proteins, AlF-C1 and AlF-C2, which repressed the AldB gene promoter. We also reported that the binding site of these proteins, site C, is one of the required DNA elements of the AldB gene origin/promoter for autonomously replicating activity in transfected cells. In the present study, we show that AlF-C1 and AlF-C2 bind directly to Orc1, a subunit of the origin recognition complex (ORC). Deletion analyses revealed a functional domain in AlF-C2 for binding to Orc1, which is located separately from the DNA-binding domain. In addition, we found a novel protein-interacting domain in Orc1 required for the binding of AlF-C2, which was conserved in human, mouse and Chinese hamster, but not in Drosophila, frog and yeast. Thus, it is assumed that in mammalian cells, sequence- specific DNA-binding proteins are involved in recruiting ORC to regulate replication initiation and/or transcription repression.
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出版者 |
en
Oxford University Press
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日付 |
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言語 |
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資源タイプ |
journal article |
出版タイプ |
VoR |
資源識別子 |
HDL
http://hdl.handle.net/2115/53742
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関連 |
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DOI
https://doi.org/10.1093/nar/gkf642
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PMID
12466545
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収録誌情報 |
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Nucleic acids research
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巻30
号23
開始ページ5205
終了ページ5212
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ファイル |
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コンテンツ更新日時 |
2023-07-26 |