| タイトル |
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en
c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
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| 作成者 |
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| アクセス権 |
open access |
| 主題 |
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MeSH
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Animals
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MeSH
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Antibodies, Monoclonal/immunology
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MeSH
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Antigens, Polyomavirus Transforming
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MeSH
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Antigens, Viral, Tumor/physiology
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MeSH
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Cell Line
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MeSH
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Cercopithecus aethiops
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MeSH
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DNA Replication/drug effects
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MeSH
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DNA, Recombinant/biosynthesis
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MeSH
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DNA, Viral/biosynthesis
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MeSH
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DNA, Viral/genetics
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MeSH
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DNA-Binding Proteins/pharmacology
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MeSH
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Humans
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MeSH
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Oncogene Proteins, Viral/physiology
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MeSH
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Plasmids
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MeSH
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Proto-Oncogene Proteins/immunology
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MeSH
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Proto-Oncogene Proteins/pharmacology
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MeSH
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Proto-Oncogene Proteins c-myc
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MeSH
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Recombinant Proteins/pharmacology
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MeSH
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Simian virus 40/genetics
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MeSH
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Transfection
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NDC
499
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| 内容注記 |
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Abstract
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Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
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| 出版者 |
en
Oxford University Press
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/54049
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| 関連 |
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isIdenticalTo
DOI
https://doi.org/10.1093/nar/15.12.4889
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PMID
3037484
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| 収録誌情報 |
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en
Nucleic acids research
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巻15
号12
開始ページ4889
終了ページ4899
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
