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タイトル	en Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops.
作成者	en Lennikov, Anton en Kitaichi, Nobuyoshi NRID 1000040431366 en Fukase, Risa en Murata, Miyuki NRID 1000050423752 en Noda, Kousuke NRID 1000090296666 en Ando, Ryo NRID 1000060399847 en Ohguchi, Takeshi NRID 1000070579599 en Kawakita, Tetsuya en Ohno, Shigeaki NRID 1000050002382 en Ishida, Susumu NRID 1000010245558
アクセス権	open access
主題	MeSH en Administration, Ophthalmic MeSH en Animals MeSH en Antioxidants/administration & dosage MeSH en Antioxidants/therapeutic use MeSH en Apoptosis/drug effects MeSH en Cells, Cultured MeSH en Cornea/cytology MeSH en Cornea/drug effects MeSH en Cornea/radiation effects MeSH en Cytoprotection MeSH en Dose-Response Relationship, Drug MeSH en Epithelial Cells/cytology MeSH en Epithelial Cells/drug effects MeSH en Epithelial Cells/radiation effects MeSH en Epithelium, Corneal/cytology MeSH en Epithelium, Corneal/drug effects MeSH en Epithelium, Corneal/radiation effects MeSH en In Situ Nick-End Labeling MeSH en Keratitis/drug therapy MeSH en Keratitis/pathology MeSH en Male MeSH en Mice MeSH en Mice, Inbred C57BL MeSH en Ophthalmic Solutions/administration & dosage MeSH en Ophthalmic Solutions/therapeutic use MeSH en Oxidative Stress/drug effects MeSH en Ultraviolet Rays MeSH en Xanthophylls/administration & dosage MeSH en Xanthophylls/therapeutic use NDC 496
内容注記	Abstract en Purpose: Ultraviolet (UV) acts as low-dose ionizing radiation. Acute UVB exposure causes photokeratitis and induces apoptosis in corneal cells. Astaxanthin (AST) is a carotenoid, present in seafood, that has potential clinical applications due to its high antioxidant activity. In the present study, we examined whether topical administration of AST has preventive and therapeutic effects on UV-photokeratitis in mice. Methods: C57BL/6 mice were administered with AST diluted in polyethylene glycol (PEG) in instillation form (15 μl) to the right eye. Left eyes were given vehicle alone as controls. Immediately after the instillation, the mice, under anesthesia, were irradiated with UVB at a dose of 400 mJ/cm2. Eyeballs were collected 24 h after irradiation and stained with H&E and TUNEL. In an in vitro study, mouse corneal epithelial (TKE2) cells were cultured with AST before UV exposure to quantify the UV-derived cytotoxicity. Results: UVB exposure induced cell death and thinning of the corneal epithelium. However, the epithelium was morphologically well preserved after irradiation in AST-treated corneas. Irradiated corneal epithelium was significantly thicker in eyes treated with AST eye drops, compared to those treated with vehicles (p<0.01), in a doses dependent manner. Significantly fewer apoptotic cells were observed in AST-treated eyes than controls after irradiation (p<0.01). AST also reduced oxidative stress in irradiated corneas. The in vitro study showed less cytotoxicity of TKE2 cells in AST-treated cultures after UVB-irradiation (p<0.01). The cytoprotective effect increased with the dose of AST. Conclusions: Topical AST administration may be a candidate treatment to limit the damages by UV irradiation with wide clinical applications.
出版者	en Molecular Vision
日付	Issued2012-02-14
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/54615
関連	PMID 22393271
収録誌情報	PISSN 1090-0535 NCID AA12037116 en Molecular vision 巻18 開始ページ455 終了ページ464
ファイル	fulltext Mol Vis_18_455-464.pdf 3.82 MB (application/pdf) Issued2012-02-14
コンテンツ更新日時	2023-07-26