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Title
  • en Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops.
Creator
Accessrights open access
Subject
  • MeSH en Administration, Ophthalmic
  • MeSH en Animals
  • MeSH en Antioxidants/administration & dosage
  • MeSH en Antioxidants/therapeutic use
  • MeSH en Apoptosis/drug effects
  • MeSH en Cells, Cultured
  • MeSH en Cornea/cytology
  • MeSH en Cornea/drug effects
  • MeSH en Cornea/radiation effects
  • MeSH en Cytoprotection
  • MeSH en Dose-Response Relationship, Drug
  • MeSH en Epithelial Cells/cytology
  • MeSH en Epithelial Cells/drug effects
  • MeSH en Epithelial Cells/radiation effects
  • MeSH en Epithelium, Corneal/cytology
  • MeSH en Epithelium, Corneal/drug effects
  • MeSH en Epithelium, Corneal/radiation effects
  • MeSH en In Situ Nick-End Labeling
  • MeSH en Keratitis/drug therapy
  • MeSH en Keratitis/pathology
  • MeSH en Male
  • MeSH en Mice
  • MeSH en Mice, Inbred C57BL
  • MeSH en Ophthalmic Solutions/administration & dosage
  • MeSH en Ophthalmic Solutions/therapeutic use
  • MeSH en Oxidative Stress/drug effects
  • MeSH en Ultraviolet Rays
  • MeSH en Xanthophylls/administration & dosage
  • MeSH en Xanthophylls/therapeutic use
  • NDC 496
Description
  • Abstract en Purpose: Ultraviolet (UV) acts as low-dose ionizing radiation. Acute UVB exposure causes photokeratitis and induces apoptosis in corneal cells. Astaxanthin (AST) is a carotenoid, present in seafood, that has potential clinical applications due to its high antioxidant activity. In the present study, we examined whether topical administration of AST has preventive and therapeutic effects on UV-photokeratitis in mice. Methods: C57BL/6 mice were administered with AST diluted in polyethylene glycol (PEG) in instillation form (15 μl) to the right eye. Left eyes were given vehicle alone as controls. Immediately after the instillation, the mice, under anesthesia, were irradiated with UVB at a dose of 400 mJ/cm2. Eyeballs were collected 24 h after irradiation and stained with H&E and TUNEL. In an in vitro study, mouse corneal epithelial (TKE2) cells were cultured with AST before UV exposure to quantify the UV-derived cytotoxicity. Results: UVB exposure induced cell death and thinning of the corneal epithelium. However, the epithelium was morphologically well preserved after irradiation in AST-treated corneas. Irradiated corneal epithelium was significantly thicker in eyes treated with AST eye drops, compared to those treated with vehicles (p<0.01), in a doses dependent manner. Significantly fewer apoptotic cells were observed in AST-treated eyes than controls after irradiation (p<0.01). AST also reduced oxidative stress in irradiated corneas. The in vitro study showed less cytotoxicity of TKE2 cells in AST-treated cultures after UVB-irradiation (p<0.01). The cytoprotective effect increased with the dose of AST. Conclusions: Topical AST administration may be a candidate treatment to limit the damages by UV irradiation with wide clinical applications.
Publisher en Molecular Vision
Date
    Issued2012-02-14
Language
  • eng
Resource Type journal article
Version Type VoR
Identifier HDL http://hdl.handle.net/2115/54615
Relation
  • PMID 22393271
Journal
    • PISSN 1090-0535
    • NCID AA12037116
      • en Molecular vision
      • Volume Number18 Page Start455 Page End464
File
Oaidate 2023-07-26