| タイトル |
-
en
Polymerase complex with lysine at position 627 of the PB2 of influenza virus A/Hong Kong/483/97 (H5N1) efficiently transcribes and replicates virus genes in mouse cells
|
| 作成者 |
|
| アクセス権 |
open access |
| 主題 |
-
Other
en
PB2
-
Other
en
H5N1
-
Other
en
Influenza A virus
-
Other
en
Polymerase
-
NDC
649
|
| 内容注記 |
-
Abstract
en
Influenza virus A/Hong Kong/483/97 (H5N1) (HK483-K) has the PB2 with lysine at position 627 (PB2-627K) and is highly pathogenic in chickens and mice. On the other hand, the pathogenicity of mutant virus (HK483-E), which was generated by substituting lysine with glutamic acid at the position of the PB2, is lower than that of HK483-K in mice, but is highly pathogenic in chickens. The PB2 is one of the components of heterotrimeric polymerase complex, which plays roles in the transcription and replication of virus genes. Cell-free polymerase assay revealed that intrinsic transcription activity of the polymerase complex with PB2-627K is higher than that of glutamic acid (PB2-627E). In chicken cells, transcription efficiency of the polymerase complex with PB2-627E was not lower than those with PB2-627K, indicating that transcription of virus genes is modulated by some host factors in chicken cells, resulting in high growth. Polymerase complex with PB2-627K efficiently transcribes and replicates virus polymerase genes in mouse cells, leading to high growth of HK483-K compared with that of HK483-E. The results of our experiments clearly suggest that efficient transcription and replication of virus genes by polymerase complex result in the higher pathogenicity in mice. (C) 2013 Elsevier B.V. All rights reserved.
|
| 出版者 |
en
Elsevier
|
| 日付 |
|
| 言語 |
|
| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/54759
|
| 関連 |
-
isVersionOf
DOI
https://doi.org/10.1016/j.virusres.2013.09.012
-
PMID
24070983
|
| 収録誌情報 |
-
-
en
Virus Research
-
巻178
号2
開始ページ404
終了ページ410
|
| ファイル |
|
| コンテンツ更新日時 |
2023-07-26 |
