| タイトル |
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en
Application of apolipoprotein E-modified liposomal nanoparticles as a carrier for delivering DNA and nucleic acid in the brain
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| 作成者 |
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| アクセス権 |
open access |
| 権利情報 |
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| 主題 |
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Other
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brain
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Other
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DNA
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Other
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oligonucleotide
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Other
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delivery
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Other
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nanoparticles
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NDC
499
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| 内容注記 |
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Abstract
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An innovative drug delivery technology is urgently needed to satisfy unmet medical needs in treating various brain disorders. As a fundamental carrier for plasmid DNA or nucleic acids, we developed a liposomal nanoparticle (multifunctional envelope-type nano device [MEND]) containing a proton-ionizable amino lipid (YSK-MEND). Here we report on the impact of apolipoprotein E (ApoE) modification on the function of YSK-MEND in terms of targeting brain cells. The cellular uptake and function of YSK-MEND encapsulating short interference RNA or plasmid DNA were significantly improved as a result of ApoE modification in mouse neuron-derived cell lines (Neuro-2a and CAD). Intracerebroventricular administration of ApoE-modified YSK-MEND (ApoE/YSK-MEND) encapsulating plasmid DNA also resulted in higher transgene expression in comparison with YSK-MEND that was not modified with ApoE. Moreover, observation of fluorescence-labeled ApoE/YSK-MEND and expression of mCherry (fluorescence protein) derived from plasmid DNA indicated that this carrier might be useful for delivering and conferring transgene expression in neural stem cells and/or neural progenitor cells. Thus, this system may be a useful tool for the treatment of neurodegenerative disease.
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| 出版者 |
en
Dove Medical Press
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/57260
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| 関連 |
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isIdenticalTo
DOI
https://doi.org/10.2147/IJN.S65402
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| 収録誌情報 |
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en
International Journal of Nanomedicine
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巻9
開始ページ4267
終了ページ4276
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
