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Title
  • Application of apolipoprotein E-modified liposomal nanoparticles as a carrier for delivering DNA and nucleic acid in the brain
Creator

Tamaru, Mina

Akita, Hidetaka

Nakatani, Taichi

Kajimoto, Kazuaki

Sato, Yusuke

Hatakeyama, Hiroto

Harashima, Hideyoshi

Rights
    • http://creativecommons.org/licenses/by-nc/3.0/us/
Subject
  • Other brain
  • Other DNA
  • Other oligonucleotide
  • Other delivery
  • Other nanoparticles
  • NDC 499
Description
Other
  • An innovative drug delivery technology is urgently needed to satisfy unmet medical needs in treating various brain disorders. As a fundamental carrier for plasmid DNA or nucleic acids, we developed a liposomal nanoparticle (multifunctional envelope-type nano device [MEND]) containing a proton-ionizable amino lipid (YSK-MEND). Here we report on the impact of apolipoprotein E (ApoE) modification on the function of YSK-MEND in terms of targeting brain cells. The cellular uptake and function of YSK-MEND encapsulating short interference RNA or plasmid DNA were significantly improved as a result of ApoE modification in mouse neuron-derived cell lines (Neuro-2a and CAD). Intracerebroventricular administration of ApoE-modified YSK-MEND (ApoE/YSK-MEND) encapsulating plasmid DNA also resulted in higher transgene expression in comparison with YSK-MEND that was not modified with ApoE. Moreover, observation of fluorescence-labeled ApoE/YSK-MEND and expression of mCherry (fluorescence protein) derived from plasmid DNA indicated that this carrier might be useful for delivering and conferring transgene expression in neural stem cells and/or neural progenitor cells. Thus, this system may be a useful tool for the treatment of neurodegenerative disease.
PublisherDove Medical Press
Date Issued 2014-09-08
Languageeng
NIItypejournal article
VersiontypeVoR
Identifier URI http://hdl.handle.net/2115/57260
Relation
  • isIdenticalTo DOI https://doi.org/10.2147/IJN.S65402
Journal
    • ISSN 1178-2013
    • International Journal of Nanomedicine
    9, 4267-4276
File
Oaidate2017-10-15T15:00:00Z