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タイトル	en Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses
作成者	en Hagiwara-Komoda, Yuka en Choi, Sun Hee en Sato, Masanao en Atsumi, Go en Abe, Junya en Fukuda, Junya en Honjo, Mie N. en Nagano, Atsushi J. en Komoda, Keisuke en Nakahara, Kenji S. NRID 1000090315606 en Uyeda, Ichiro NRID 1000010113523 en Naito, Satoshi NRID 1000020164105
アクセス権	open access
権利情報	http://creativecommons.org/licenses/by/4.0/ en Creative Commons Attribution 4.0 International
内容注記	Abstract en RNA viruses use various strategies to condense their genetic information into small genomes. Potyviruses not only use the polyprotein strategy, but also embed an open reading frame, pipo, in the P3 cistron in the -1 reading frame. PIPO is expressed as a fusion protein with the N-terminal half of P3 (P3N-PIPO) via transcriptional slippage of viral RNA-dependent RNA polymerase (RdRp). We herein show that clover yellow vein virus (ClYVV) produces a previously unidentified factor, P3N-ALT, in the +1 reading frame via transcriptional slippage at a conserved G(1-2)A(6-7) motif, as is the case for P3N-PIPO. The translation of P3N-ALT terminates soon, and it is considered to be a C-terminal truncated form of P3. In planta experiments indicate that P3N-ALT functions in cell-to-cell movement along with P3N-PIPO. Hence, all three reading frames are used to produce functional proteins. Deep sequencing of ClYVV RNA from infected plants endorses the slippage by viral RdRp. Our findings unveil a virus strategy that optimizes the coding capacity.
出版者	en Nature Publishing Group
日付	Issued2016-02-22
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/61175
関連	isIdenticalTo DOI https://doi.org/10.1038/srep21411
収録誌情報	PISSN 2045-2322 en Scientific reports 巻6 開始ページ21411
ファイル	fulltext srep21411.pdf 1.49 MB (application/pdf) Issued2016-02-22
コンテンツ更新日時	2023-07-26