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Title	en Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses
Creator	en Hagiwara-Komoda, Yuka en Choi, Sun Hee en Sato, Masanao en Atsumi, Go en Abe, Junya en Fukuda, Junya en Honjo, Mie N. en Nagano, Atsushi J. en Komoda, Keisuke en Nakahara, Kenji S. NRID 1000090315606 en Uyeda, Ichiro NRID 1000010113523 en Naito, Satoshi NRID 1000020164105
Accessrights	open access
Rights	http://creativecommons.org/licenses/by/4.0/ en Creative Commons Attribution 4.0 International
Description	Abstract en RNA viruses use various strategies to condense their genetic information into small genomes. Potyviruses not only use the polyprotein strategy, but also embed an open reading frame, pipo, in the P3 cistron in the -1 reading frame. PIPO is expressed as a fusion protein with the N-terminal half of P3 (P3N-PIPO) via transcriptional slippage of viral RNA-dependent RNA polymerase (RdRp). We herein show that clover yellow vein virus (ClYVV) produces a previously unidentified factor, P3N-ALT, in the +1 reading frame via transcriptional slippage at a conserved G(1-2)A(6-7) motif, as is the case for P3N-PIPO. The translation of P3N-ALT terminates soon, and it is considered to be a C-terminal truncated form of P3. In planta experiments indicate that P3N-ALT functions in cell-to-cell movement along with P3N-PIPO. Hence, all three reading frames are used to produce functional proteins. Deep sequencing of ClYVV RNA from infected plants endorses the slippage by viral RdRp. Our findings unveil a virus strategy that optimizes the coding capacity.
Publisher	en Nature Publishing Group
Date	Issued2016-02-22
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/61175
Relation	isIdenticalTo DOI https://doi.org/10.1038/srep21411
Journal	PISSN 2045-2322 en Scientific reports Volume Number6 Page Start21411
File	fulltext srep21411.pdf 1.49 MB (application/pdf) Issued2016-02-22
Oaidate	2023-07-26