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タイトル	en Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation
作成者	en Kimura, Shunsuke NRID 1000040444525 en Yamashita, Masami en Yamakami-Kimura, Megumi en Sato, Yusuke en Yamagata, Atsushi NRID 1000020463903 en Kobashigawa, Yoshihiro en Inagaki, Fuyuhiko NRID 1000070011757 en Amada, Takako en Hase, Koji NRID 1000020359714 en Iwanaga, Toshihiko NRID 1000010160128 en Ohno, Hiroshi NRID 1000050233226 en Fukai, Shuya NRID 1000010361792
アクセス権	open access
権利情報	https://creativecommons.org/licenses/by/4.0/ en Creative Commons Attribution 4.0 International
主題	NDC 490
内容注記	Abstract en The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.
出版者	en Nature Publishing Group
日付	Issued2016-09-15
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/63450
関連	isIdenticalTo DOI https://doi.org/10.1038/srep33548
収録誌情報	PISSN 2045-2322 en Scientific reports 巻6 開始ページ33548
ファイル	fulltext srep33548.pdf 1.64 MB (application/pdf) Issued2016-09-15
コンテンツ更新日時	2023-07-26